prikaz prve stranice dokumenta Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles
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master's thesis
Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles
Split: University of Split, University of Split, Faculty of science, 2019. urn:nbn:hr:166:883914
Kovač, Dubravka
University of Split
Faculty of Science
Department of Physics

Institutional repository: Repository of Faculty of Science

Cite this document

Kovač, D. (2019). Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:883914

Kovač, Dubravka. "Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles." Master's thesis, University of Split, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:166:883914

Kovač, Dubravka. "Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles." Master's thesis, University of Split, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:166:883914

Kovač, D. (2019). 'Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles', Master's thesis, University of Split, Faculty of Science, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:883914

Kovač D. Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles [Master's thesis]. Split: University of Split, Faculty of Science; 2019 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:166:883914

D. Kovač, "Cholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles", Master's thesis, University of Split, Faculty of Science, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:166:883914

Metadata
TitleCholesterol content and AC-electric field impact on electroformation of giant unilamellar vesicles
Title (croatian)Utjecaj kolesterola i izmjeničnog električnog polja na elektroformaciju ogromnih unilamelarnih vezikula
AuthorDubravka Kovač
MentorMarija Raguž (mentor)
Committee memberMarija Raguž (predsjednik povjerenstva)
Committee memberAnte Bilušić (član povjerenstva)
Committee memberLarisa Zoranić (član povjerenstva)
GranterUniversity of Split
Faculty of Science
(Department of Physics)
Split
Defense date and country2019-07-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Scientists have been trying to understand processes in cell membranes for a long time. So far, giant unilamellar vesicles (GUVs) are used as models for biological membranes because of their lipid composition similarity and their potential as drug carriers. In this study, we created GUVs using electroformation method. Our lipid solution contained one-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and cholesterol-3 β -cholest-5-en-3-ol (Chol) with different mixing ratios and as a fluorescent dye we used 1,1'-dioctadecyl-3,3,3'3'-tetramethylin docarbocyanine perchlorate (DiI). In order to determine best parameters for electroformation we conducted set of experiments with different frequency-field strength combinations: 10-10000 Hz and 0.625-9.375 V/mm. We founded that optimal values are in the ranges around 10-100 Hz and 1.25-3.75 V/mm. Additionally, the experiments that we conducted with different cholesterol concentrations show that increasing of cholesterol content decreases GUVs size.
Abstract (croatian)
Znanstvenici pokušavaju razumjeti procese unutar staničnih membranama već dugo vremena. Zasad su se ogromne unilamelarne vezikule (GUVs) koristile kao modeli za biološke membrane zbog slične lipidne kompozicije kao i njihovog potencijala za unos lijekova. U ovom istraživanju GUV-ove smo stvorili koristeći elektroformacijsku metodu. Naša lipidna otopina sadrži jedan-palmitoil-2-oleoil-glicero-3-fosfokolin (POPC) i kolesterol-3 β-kolest-5en-3-ol (Chol) u različitim omjerima, dok kao fluorescentnu boju koristimo.1,1 '-dioktadecil-3,3,3'3'-tetrametilin dokarbocianin perklorat (DiI). Kako bi smo odredili najbolje parametre za elektroformaciju, proveli smo set eksperimenata s različitim kombinacijama frekvencije i jačine polja: 10-10000 Hz i 0.625-9.375 V / mm. Pronašli smo da se optimalne vrijednosti kreću oko 10-100 Hz i 1,25-3,75 V / mm. Dodatno, eksperimenti koje smo proveli s različitim koncentracijama kolesterola pokazuje da povećannje udjela kolesterola smanjuje veličinu GUV-ova.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:166:883914
Study programmeTitle: Physics; specializations in: major in teaching, major in Astrophysics, major in Biophysics, major in Enviromental physics, major in Computational physics
Course: major in Computational physics
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-11-22 09:38:10