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master's thesis
Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. urn:nbn:hr:109:145977
Sabljić, Marija
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Sub-department of Applied Chemistry and instrumental Methods
Instrumental Methods II

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Sabljić, M. (2016). Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:145977

Sabljić, Marija. "Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. https://urn.nsk.hr/urn:nbn:hr:109:145977

Sabljić, Marija. "Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. https://urn.nsk.hr/urn:nbn:hr:109:145977

Sabljić, M. (2016). 'Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:109:145977

Sabljić M. Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2016 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:109:145977

M. Sabljić, "Validacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:109:145977

Metadata
TitleValidacija spektroskopskih i kromatografskih metoda za određivanje polifenolnih spojeva
Title (english)Validation of spectroscopic and chromatographic methods for polyphenolic compound determination
AuthorMarija Sabljić
MentorLidija Jakobek (mentor)
Committee memberDaniela Čačić Kenjerić (predsjednik povjerenstva)
Committee memberLidija Jakobek (član povjerenstva)
Committee memberIvana Flanjak (član povjerenstva)
Committee memberMirna Habuda-Stanić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Sub-department of Applied Chemistry and instrumental Methods)
(Instrumental Methods II)
Osijek
Defense date and country2016-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
U ovom radu provedena je validacija spektroskopskih metoda (metoda za ukupne polifenole (Folin-Ciocalteu metoda), metoda za ukupne flavonoide, metoda za ukupne antocijanine (pH-diferencijalna metoda)) i kromatografske metode za određivanje polifenolnih spojeva. Parametri validacije koji su se određivali bili su: linearnost, granica detekcije, granica kvantifikacije, preciznost, točnost te osjetljivost. Folin Ciocalteu metoda validirana je za galnu kiselinu, (+)-katehin i kvercetin-3-rutinozid, metoda za ukupne flavonoide za (+)-katehin, kvercetin-3-rutinozid i kvercetin, a metoda za ukupne antocijanine za cijanidin-3-galaktozid i cijanidin-3-glukozid. Kromatografska metoda validirana je za sve spomenute polifenole. Utvrđeno je da su spektroskopske metode linearne, s prihvatljivim granicama detekcije i kvantifikacije te s prihvatljivom preciznošću i točnošću. Osjetljivost je bila najbolja za standarde polifenola koji se uobičajeno koriste kao standardi: galna kiselina za ukupne polifenole, (+)-katehin za ukupne flavonoide, cijanidin-3-glukozid za ukupne antocijanine. Kromatografska metoda je također dovoljno precizna i točna, s dobrim granicama detekcije, kvantifikacije, linearnošću te s prihvatljivom osjetljivošću.
Abstract (english)
In this paper, the validation of spectroscopic methods (method for total polyphenols (Folin-Ciocalteu method), method for total flavonoids, method for total anthocyanins (pH differential method)) and chromatographic method for the determination of polyphenol compounds was carried out. Validation parameters which were determined were: linearity, limit of detection, limit of quantification, precision, accuracy and sensitivity. Folin-Ciocalteu method was validated for gallic acid, (+) - catechin and quercetin-3-rutinoside, methods for the total flavonoid content for (+) - catechin, quercetin-3-rutinoside and quercetin and method for total anthocyanins for cyanidin-3- galactoside and cyanidin-3-glucoside. Chromatographic method was validated for all of these polyphenols. It has been shown that spectroscopic methods are linear, with acceptable limits of detection and quantification and with acceptable precision and accuracy. The sensitivity was the best for the polyphenol standards which are commonly used: gallic acid for total polyphenols, (+) - catechin for total flavonoids, cyanidin-3-glucoside for total anthocyanins. Chromatographic method was also sufficiently precise and accurate, with good limits of detection, quantification, linearity and with reasonable sensitivity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:145977
Study programmeTitle: Food Science and Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani (magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-09-20 14:25:16