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master's thesis
Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. urn:nbn:hr:109:025088
Strugačevac, Livia
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Strugačevac, L. (2015). Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:025088

Strugačevac, Livia. "Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:025088

Strugačevac, Livia. "Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:025088

Strugačevac, L. (2015). 'Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:109:025088

Strugačevac L. Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2015 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:109:025088

L. Strugačevac, "Usporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:109:025088

Metadata
TitleUsporedba ELISA i LC-MS/MS tehnike u analizi aflatoksina u kukuruzu
Title (english)Comparison of ELISA and LC-MS/MS techniques in aflatoxin analysis in maize
AuthorLivia Strugačevac
MentorTomislav Klapec (mentor)
Committee memberDaniela Čačić Kenjerić
Committee memberTomislav Klapec
Committee memberNatalija Velić
Committee memberJelka Pleadin
MBZ: 273891
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2015-01-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Aflatoksini su toksični sekundarni metaboliti plijesni Aspergillus spp. Cilj rada bio je usporediti pozitivne rezultate aflatoksina dobivene ELISA metodom s potvrdnom LC-MS/MS metodom. Ispitivanje je provedeno na kukuruzu, berbe 2012. godine u kojoj je prevladavalo vrlo toplo i suho vrijeme, a uzorci su sakupljeni 2013. godine na području istočne Slavonije. LC-MS/MS metoda je validirana uz iskorištenje od 79 do 96 %, te limit detekcije od 0,5 do 1 μg kg-1 i limit kvantifikacije od 1,65 do 3,3 μg kg-1. Rezultati s LC-MS/MS metodom pokazali su sljedeće omjere B1:B2:G1:G2 aflatoksina: 1:0,08:0,18:0,02. Najviša vrijednost dobivena LC-MS/MS-om odnosi se na aflatoksin B1 i iznosi 233,57 μg kg-1, dok je najveća vrijednost utvrđena ELISA testom za navedeni aflatoksin bila 1710,03 μg kg-1. Uspoređujući metode, rezultati dobiveni ELISA-om bili su prosječno viši za 364 μg kg-1 u odnosu na rezultate dobivene na LC-MS/MS-u. Iz svega navedenog može se zaključiti prednost ELISA-e kao metode za praćenje zbog brzih rezultata i detekcije prisustva aflatoksina u uzorku, no rezultati se moraju potvrditi nekom preciznijom metodom, što je u ovom slučaju bila LC-MS/MS metoda.
Abstract (english)
Aflatoxins are toxic secondary metabolites of Aspergillus spp. The aim of this study was to compare the positive results of aflatoxins obtained by ELISA assay with the confirmatory LC-MS/MS method. Analysis was performed on maize, harvested in 2012 which was characterized by very hot and dry weather, and the samples were collected in 2013 in eastern Slavonia. The LC-MS/MS method was validated with recovery range from 79 to 96 %, detection limit from 0.5 to 1 μg kg-1 and limit quantification from 1.65 to 33 μg kg-1. The LC-MS/MS results showed the following aflatoxin B1:B2:G1:G2 ratio: 1:0.08:0.18:0.02. The highest value obtained by LC–MS/MS refers to 233.57 μg kg-1 aflatoxin B1, while the highest aflatoxin B1 value obtained by ELISA was 1710.03 μg kg-1. The results obtained by ELISA were higher for 364 ug kg-1 on average compared to the LC-MS/MS results. Taken together, the results point to an advantage of ELISA assay due to its applicability for rapid screening and detection of aflatoxin presence. However, the results have to be confirmed using a more precise technique, which in this case was the LC-MS/MS method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:025088
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2015-09-28 10:17:49