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professional thesis
Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. urn:nbn:hr:109:039459
Cvejić, Jelena Helen
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Nutrition

Institutional repository: Repository of the Faculty of Food Technology Osijek

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Cvejić, J. H. (2021). Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora (Professional thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:039459

Cvejić, Jelena Helen. "Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora." Professional thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. https://urn.nsk.hr/urn:nbn:hr:109:039459

Cvejić, Jelena Helen. "Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora." Professional thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. https://urn.nsk.hr/urn:nbn:hr:109:039459

Cvejić, J. H. (2021). 'Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora', Professional thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 30 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:039459

Cvejić JH. Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora [Professional thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2021 [cited 2024 April 30] Available at: https://urn.nsk.hr/urn:nbn:hr:109:039459

J. H. Cvejić, "Protuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora", Professional thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:109:039459

Metadata
TitleProtuupalna snaga kombinacije omega-3 masnih kiselina i flavonoida i perspektiva u ublažavanju simptoma umora i sindroma kroničnog umora
Title (english)The anti-inflammatory power of the combination of omega-3 fatty acids and flavonoids and perspectives in relieving fatigue symptoms and chronic fatigue syndrome
AuthorJelena Helen Cvejić
MentorTomislav Klapec (mentor)
MentorIvana Beara https://orcid.org/0000-0002-7587-957X (pomoć pri izradi, neposredni voditelj)
Committee memberMarina Tišma (predsjednik povjerenstva)
Committee memberTomislav Klapec (član povjerenstva)
Committee memberInes Banjari (član povjerenstva)
Committee memberIvana Tomac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Food and Nutrition Research)
(Sub-department of Nutrition)
Osijek
Defense date and country2021-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Nutritional Science
Abstract
Dugotrajni nakupljeni umor utječe na tjelesno i mentalno zdravlje ljudi, istodobno uzrokujući razne bolesti, uključujući sindrom kroničnog umora (CFS). Gotovo 40% cjelokupne populacije pati od značajnog stepena umora. Istraživanja ukazuju na vezu između proupalnog statusa i oksidacijskog stresa s pojavom kroničnog umora, dok sve veći broj studija ukazuje na terapijski potencijal protuupalnih i antioksidativnih sastojaka hrane. Cilj ovog rada je procjena utjecaja odabranih flavonoida i metabolita (apigenin, apigenin-7-O-glukozid, luteolin, luteolin-7-O-glukozid, apigenin-7-O-glukuronid, luteolin-7-O-glukuronid) te omega-3 masnih kiselina (dokosaheksaenska kiselina i eikosapentaenska kiselina) na proizvodnju upalnih medijatora (PLA2, COX-1, COX-2, mPGES-1, mPGES-2, cPGES, TXAS) na modelu rane upale, kao i ispitvanje ekspresije mRNA enzima cPLA2α, COX-1/2, mPGES-1/2, cPGES i TXAS uključenih u njihovu proizvodnju uz RT-qPCR. Smjese flavonoida s omega-3 masnim kiselinama, ovisno o primijenjenim koncentracijama i kombinacijama, inhibiraju ili potenciraju stvaranje PGE2 i TXA2 (kvantificiranih ELISA testom) u monocitima U937 stimuliranim lipopolisaharidom (LPS). Iste smjese, osim onih s luteolinom i luteolin-7-O-glukuronidom, povećavaju ekspresiju TXAS mRNA, ali bez izravne povezanosti s količinom proizvedenog TXA2. Najprominentniji protuupalni potencijal pokazuje kombinacija masnih kiselina s luteolinom, odnosno njegovim metabolitom, što ukazuje na racionalnu osnovu za njihovo uključivanje u daljnje faze istraživanja i testiranje terapijskog učinka u ublažavanju simptoma umora i CFS-a.
Abstract (english)
Prolonged accumulated fatigue threatens people’s physical and mental health, while causing various illnesses, including chronic fatigue syndrome (CFS). Almost 40% of the total population suffers from pathological fatigue. A link between pro-inflammatory status and oxidative stress with the onset of chronic fatigue, and diet significance are indicated. The aim of this study was to evaluate the influence of selected flavonoids and metabolites (apigenin, apigenin-7-O-glucoside, luteolin, luteolin-7-O-glucoside, apigenin-7-O-glucuronide, luteolin-7-O-glucuronide), and omega-3 fatty acids on the inflammatory mediators (PLA2, COX-1, COX-2, mPGES-1, mPGES-2, cPGES, TXAS) production in an early inflammation model and to examine the expression of corresponding mRNA enzymes cPLA2α, COX-1/2, mPGES-1/2, cPGES i TXAS (RT-qPCR). Mixtures of flavonoids with omega-3 fatty acids, depending on the applied concentrations and combinations, inhibit or potentiate the production of PGE2 and TXA2 (quantified by ELISA test) in LPS-stimulated U937 monocytes. The same mixtures, excluding the ones with luteolin and luteolin-7-O-glucuronide, increase the expression of TXAS mRNA without a direct relationship to the amount of TXA2 produced. The most prominent anti-inflammatory potential is shown by the combinations of fatty acids with luteolin and its metabolite, indicating a rational basis for their inclusion in further research and for testing their therapeutic effect in relieving symptoms of fatigue and CFS.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:039459
Study programmeTitle: Nutrition
Study programme type: university
Study level: postgraduate specialist
Academic / professional title: sveučilišni/a specijalist/specijalistica nutricionizma (sveučilišni/a specijalist/specijalistica nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Public notepomoć pri izradi ovog rada bila je prof. dr. sc Ivana Beara s Prirodno-matematičkog fakulteta Sveučilišta u Novom Sadu
Created on2021-10-27 07:46:50