This study tested three white rot fungi (four strains) P. chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 and C. subvermispora for their ability to decolorize five synthetic dyes (Congo Red, Malachite Green, Crystal Violet, Fuchsine and Methylene Blue). The fungi were cultivated at 27 ⁰C for 9 (10) days on agar plates containing dyes at final concentrations of 50, 100 and 150 mg L-1. The radial growth and the zone of colour change on the agar plates were measured regularly every three days. Both T. versicolor strains, as well C. subvermispora were able to decolorize azo dye Congo Red to some extent. Congo Red did not inhibit the mycelial growth of investigated fungi. Malachite Green strongly inhibited the mycelial growth of all fungal strains. However, high decolourization index (decolourization diameter/mycelial diameter) ranging from 2.42 to 6.04 for all species indicates very good decolourization ability. All fungi were able to decolorize Crystal Violet to some extent. However, apart from P. chrysosporium, growth of all other strains was strongly inhibited by this dye. Fuchsine inhibited the mycelial growth only during the first days of cultivation. All strains were able to decolorize fuchsine, but not as effectively as other two dyes of triphenylmethane group (decolourization index ranging from 0.24 to 1). Only T. versicolor TV6 decolorized Methylene Blue to some extent, even though this dye did not inhibit the mycelial growth of the tested fungal strains. The influence of agar plate dye concentration on fungal growth was statistically significant, while no statistical significance was observed regarding the decolourization ability.