Mn, Al and Ni), and of some potential contaminants (Pb and Cd) was determined in 200 samples of the black locust honey, which were collected during 2009 and 2010 in five Croatian regions (Varaždin, Krapina-Zagorje, Bjelovar-Bilogora, eastern Croatia, and Istria regions), as well as in 100 samples of soil of the area the apiaries were placed in. This was preceded by the melissopalynological and physicochemical analysis of honey, which confirmed the botanical origin of honey, and it determined that 191 samples meet the general quality requirements. By statistically analyzing the data, an attempt was made to point out the differences in mineral composition of honey with respect to its geographical origin. Applying the analysis of variance to the data on the portion of all twelve minerals no significant statistical difference was found in the portion of minerals with respect to the region (p > 0.05), while taking the season into consideration, the analysis showed the statistical difference only for Cd (p > 0.05). Regression analysis revealed a mutual relationship of minerals’ portions in the soil and honey only between Na and K, which is described in the best manner by linear function and is characterized by high correlation coefficients. Following the analysis of main components, the potential minerals which are characteristic of the black locust honey are as follows: Al (Bjelovar-Bilogora region), Fe (Bjelovar-Bilogora and Istria region), Cu (eastern Croatia region) and K (Istria region).