prikaz prve stranice dokumenta Metabolizam deoksinivalenola i analiza njegovih biomarkera
  Download
PDF 1.36 MB
master's thesis
Metabolizam deoksinivalenola i analiza njegovih biomarkera
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. urn:nbn:hr:109:301649
Podravac, Dijana
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Nutrition

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Podravac, D. (2016). Metabolizam deoksinivalenola i analiza njegovih biomarkera (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:301649

Podravac, Dijana. "Metabolizam deoksinivalenola i analiza njegovih biomarkera." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. https://urn.nsk.hr/urn:nbn:hr:109:301649

Podravac, Dijana. "Metabolizam deoksinivalenola i analiza njegovih biomarkera." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2016. https://urn.nsk.hr/urn:nbn:hr:109:301649

Podravac, D. (2016). 'Metabolizam deoksinivalenola i analiza njegovih biomarkera', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:109:301649

Podravac D. Metabolizam deoksinivalenola i analiza njegovih biomarkera [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2016 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:109:301649

D. Podravac, "Metabolizam deoksinivalenola i analiza njegovih biomarkera", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:109:301649

Metadata
TitleMetabolizam deoksinivalenola i analiza njegovih biomarkera
Title (english)Deoxynivalenol metabolism and analysis of its biomarkers
AuthorDijana Podravac
MentorBojan Šarkanj (mentor)
Committee memberTomislav Klapec (predsjednik povjerenstva)
Committee memberBojan Šarkanj (član povjerenstva)
Committee memberDaniela Čačić Kenjerić (član povjerenstva)
Committee memberIvana Flanjak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Food and Nutrition Research)
(Sub-department of Nutrition)
Osijek
Defense date and country2016-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Food Microbiology
Abstract
Mikotoksini su sekundarni metaboliti plijesni koje nastaju na polju ili tijekom skladištenja uroda te često kontaminiraju hranu. Deoksinivalenol (DON) i njegovi maskirani oblici su mikotoksini koje proizvode plijesni vrste Fusarium i najčešće kontaminiraju kukuruz, pšenicu, ječam i zob. Zbog svojeg štetnog utjecaja na zdravlje, potrebno je raditi na razvoju novih metoda njegovog određivanja kako bi se olakšala procjena rizika i procjena izloženosti. U ovom radu je istražena stopa izlučivanja i glukuronidacije DON-a pomoću biomarkera iz urina 49 dobrovoljaca nakon konzumacije namirnica s poznatim koncentracijama DON-a i njegovih metabolita. Za analizu urina korištena je multimikotoksinska metoda ultra visokodjelotvorne tekućinske kromatografije – tandemske masene spektrometrije (UHPLC-MS/MS). Analiza prvog jutarnjeg urina nakon dva dana pripreme za intervencijsku prehranu provedena je da bi se potvrdilo nepostojanje DON-a u urinu ispitanika koji bi mogao utjecati na rezultate prehrane jer se DON eliminira iz organizma unutar 24 sata. 24-satni urin trećeg intervencijskog dana potvrdio je da se većina DON-a izluči u obliku DON-15-glukuronida, nešto manje u slobodnom obliku, a ostatak u obliku DON-3-glukuronida koji kod nekih ispitanika nije bio ni detektiran zbog preniskih koncentracija DON-a u unesenoj hrani. Stopa izlučivanja bila je 82%, a stopa konjugacije 89 %.
Abstract (english)
Mycotoxins are secondary metabolites of molds that occur in the field or during storage of crops and often contaminate food. Deoxynivalenol (DON) and its masked forms are mycotoxins produced by Fusarium molds and commonly contaminate corn, wheat, barley and oats. Due to its harmful effects on health, it is necessary to work on the development of new methods of determining how to facilitate exposure and risk assessment. This work investigated the rate of excretion and glucuronidation of DON using biomarkers from urine of 49 volunteers after they consumed foods with known concentrations of DON and its metabolites. Multi-mycotoxin ultra high-performance liquid chromatography - tandem mass spectrometry (UHPLC-MS / MS) method was used for urine analysis. Analysis of the first morning urine after two days of preparation for nutritional intervention was carried out to confirm absence of DON in the urine of subjects that could affect the results of the intervention because DON is eliminated from the body within 24 hours. 24-hour urine from the third intervention day confirmed that the majority of DON was excreted in the form of DON-15-glucuronide, a little less in the free form, and the rest in the form of DON-3-glucuronide which was not detected in some subjects because of low DON concentrations in their food. The rate of excretion and conjugation were 82% and 89%, respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:301649
Study programmeTitle: Food Science and Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani (magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-07-20 07:16:25