prikaz prve stranice dokumenta GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples
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Scientific paper - Original scientific paper
GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples
International Journal of Molecular Sciences, 25 (2024), 11; 6084. https://doi.org/10.3390/ijms25116084
Kurtović, Matea; Piteša, Nikolina; Čonkaš, Josipa; Hajpek, Helena; Vučić, Majda; Musani, Vesna; Ozretić, Petar; Sabol, Maja

Institutional repository: University of Zagreb School of Dental Medicine Repository

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Kurtović, M., Piteša, N., Čonkaš, J., Hajpek, H., Vučić, M., Musani, V. ... Sabol, M. (2024). GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples. International Journal of Molecular Sciences, 25. (11). doi: 10.3390/ijms25116084

Kurtović, Matea, et al. "GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples." International Journal of Molecular Sciences, vol. 25, no. 11, 2024. https://doi.org/10.3390/ijms25116084

Kurtović, Matea, Nikolina Piteša, Josipa Čonkaš, Helena Hajpek, Majda Vučić, Vesna Musani, Petar Ozretić and Maja Sabol. "GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples." International Journal of Molecular Sciences 25, no. 11 (2024). https://doi.org/10.3390/ijms25116084

Kurtović, M., et al. (2024) 'GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples', International Journal of Molecular Sciences, 25(11). doi: 10.3390/ijms25116084

Kurtović M, Piteša N, Čonkaš J, Hajpek H, Vučić M, Musani V, and sur.. GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples. International Journal of Molecular Sciences [Internet]. 2024 May 31 [cited 2024 July 14];25(11). doi: 10.3390/ijms25116084

M. Kurtović, et al., "GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples", International Journal of Molecular Sciences, vol. 25, no. 11, May 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:127:880572. [Accessed: 14 July 2024]

Metadata
Title (english)GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples
AuthorMatea Kurtović
AuthorNikolina Piteša
AuthorJosipa Čonkaš
AuthorHelena Hajpek
AuthorMajda Vučić
AuthorVesna Musani
AuthorPetar Ozretić
AuthorMaja Sabol
Author's institutionRuđer Bošković Institute
Author's institution(Sestre milosrdnice University Hospital Center)
Author's institutionUniversity of Zagreb
School of Dental Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Although not completely understood, the role of the Hedgehog-GLI (HH-GLI) signaling pathway in melanoma and epithelial skin tumors has been reported before. In this study, we confirmed in various melanoma cell line models that keratin 16 (KRT16) and S100 Calcium-Binding Protein A7 (S100A7) are transcriptional targets of GLI Family Zinc Finger (GLI) proteins. Besides their important role in protecting and maintaining the epidermal barrier, keratins are somehow tightly connected with the S100 family of proteins. We found that stronger expression of KRT16 indeed corresponds to stronger expression of S100A7 in our clinical melanoma samples. We also report a trend regarding staining of GLI1, which corresponds to stronger staining of GLI3, KRT16, and S100A7 proteins. The most interesting of our findings is that all the proteins are detected specifically in the epidermis overlying the tumor, but rarely in the tumor itself. The examined proteins were also not detected in the healthy epidermis at the edges of the sample, suggesting that the staining is specific to the epidermis overlaying the tumor mass. Of all proteins, only S100A7 demonstrated a statistically significant trend regarding tumor staging and staining intensity. Results from our clinical samples prove that immune infiltration is an important feature of melanoma. Pigmentophages and tumor-infiltrating lymphocytes (TIL) demonstrate a significant association with tumor stage, while mononuclear cells are equally present in all stages. For S100A7, we found an association between the number of TILs and staining intensity. Considering these new findings presented in our study, we suggest a more detailed examination of the possible role of the S100A7 protein as a biomarker in melanoma.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 25, no. 11, 6084
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms25116084
URN:NBNurn:nbn:hr:127:880572
Publication2024-05-31
Document URLhttps://www.mdpi.com/1422-0067/25/11/6084
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2024-06-27 08:41:13