Tooth autotransplantation is a surgical technique which includes transferring a completely developed tooth or tooth germ from one location in the alveolar process to another with the same person. It is often used as a replacement of lost or missing teeth in young growing patients, when implant and prosthetic therapy are contraindicated. The donor tooth is usually a less important tooth, such as the third molar, a premolar or a supernumerary tooth. It is essential to preserve Hertwig's epithelial root sheath during the operative process in order to ensure root growth and development. Tooth manipulation should be as short as possible, and the surgeon should not touch the root in order to preserve the vitality of periodontal ligament cells. After the autotransplantation an endodontic procedure should be considered, and during each check-up it is necessary to monitor X-rays, vitality, position and mobility, the sound of percussion, gingival condition and probing depth. Pulp necrosis, ankylosis and supstitutional tooth resorption are signs of failure. Given the great progress of regenerative surgery in the last twenty years thanks to the use of growth factors, it is now possible to improve periodontal ligament healing and dental pulp revascularization in a transplanted tooth. One of these technologies is PRGF-Endoret, the most advanced autologous and biocompatible technological system for producing plasma rich in growth factors from the patient's autologous blood. It is based on the activation of the patient's platelets in order to stimulate accelerated regeneration and tissue healing. Endogenous proteins affect the tissue regeneration process, stimulating angiogenesis, chemotaxis and cell proliferation. The result is an accelerated healing of the soft and hard tissue and the lack of infection and postoperative pain. Tooth autotransplantation combined with plasma rich in growth factors is a major alternative to dental implants in certain situations.