The traditional chemical-mechanical procedures for cleaning the root canal are not completely successful in disinfection of endodontic space, which is mainly manifested when canal irregularities are present. Although this method removes the majority of microorganisms, some of them generally survive, and the use of additional antimicrobial agents is necessary for the success of the entire endodontic treatment and avoiding potential subsequent complications. The latest technological solutions available from several areas are being used for this purpose. One of them is the use of lasers in the disinfection and cleaning of the root canal. Advances in laser technology in the 90s have contributed to development of flexible fiber optic extensions, which enable broader use of lasers in endodontics. There are several areas of clinical application of lasers in endodontics (dentin hypersensitivity, diagnosis of the condition of the pulp, trepanation of pulp chamber, root canal filling, endodontic surgery). Lasers which are mostly used in endodontic purposes are Nd: YAG laser, diode laser and erbium laser. Although the development of lasers has progressed significantly in recent years, lasers still can not be used as the main tool when cleaning and disinfecting root canals, but that role still belongs to traditional chemical-mechanical methods. To make use of lasers in endodontics superior to the traditional methods, further research and studies in the scientific field of endodontics are necessary, as well as further advancements in laser technology.