Dental age, race, and gender estimation based on dental features is an area that forensic odontology deals with. Resistance and uniqueness of teeth make them useful in creating profiles of unknown individuals. The dental age of deceased people is most commonly estimated in the mass disaster scenarios. The need to assess the age of the living is increasing due to the migration crisis. Regarding the dental age estimation for children, useful parameters include changes that occur during tooth development and tooth eruption, while in adults and older people the important changes are the ones that occur as a result of aging. Dental age estimation methods include radiological, histological, and biochemical methods. Certain dental characteristics are more common among people who belong to the same race. However, as a result of population migration and race-mixing, these characteristics become less apparent and demand caution in forensic odontology. In forensic anthropology, races are classified into four basic groups: Caucasoids, Negroids, Mongoloids, and Australoids. Gender determination of an unknown individual demands knowledge about differences in the phenotype of males and females. Those differences are called sexual dimorphism and they refer to the fact that the teeth of males and females have different quantitative and qualitative characteristics. Isolating DNA from a tooth is the most accurate method for gender determination of an unknown person. Among the methods available for creating a dental profile, it is important to choose a method that is the most accurate in a certain situation. The accuracy of age, race, and gender estimation depends on how precise the chosen method is, on the dental forensic’s knowledge, and the multidisciplinary approach as well.