Introduction: For many years dental professionals have been using dental photography for diagnostic and treatment planning, documentation, publishing, education, marketing and communication with patients, dental technicians and colleagues. More recently, dental photography became very important for detecting facial pattern changes after orthodontic treatment, maxillofacial surgery, prosthetic oral rehabilitation and digital smile planning. Digital Single Lens Reflex (DSLR) cameras are considered as the “golden standard” cameras used to take high quality dental photographs. Past few years smartphone cameras have advanced in terms of sensor quality, resolution and lens sophistication and mobile dental photography is becoming more and more popular among dentists. Digital shade measurement determining tooth color was developed in order to reduce and overcome the inaccuracies and inconsistencies of traditional methods of tooth shade determination. Studies have shown that spectrophotometers are among the most accurate instruments for tooth color matching. Spectrophotometer Vita Easyshade V has become a reference device for determining tooth color in numerous clinical studies and has been used to determine the color of various dental materials. Numerous studies have confirmed the accuracy and repeatability of color measurements of these devices. Aim: The purpose of the study was to compare the differences in the values of the three color parameters of the upper right central incisors measured by a spectrophotometer, and calibrated and uncalibrated RAW and JPEG digital photos taken with three smartphones using a Smile Lite MDP device. Participants and methods: The study was conducted on 45 subjects. This study included those subjects with intact permanent upper central incisor without fillings and caries, endodontic procedures and prosthetic replacements, the presence of periodontal disease or any form of discoloration, and those who do not have fixed orthodontic retainers and have not undergone teeth whitening therapy for a year. The measurement took place in the research room without the natural light source (no windows), with fluorescent lighting (4 x 120 cm, 36 W, color 765, Philips, Hamburg, Germany), light temperature of 5080 K and illuminance of 500 lux measured using colorimeter Chroma-2. At each measurement infection control shield was placed over the probe tip when using Easyshade V spectrophotometer set in “tooth single” mode. The probe tip was positioned on the surface of the central area of the middle third of each right maxillary central incisor and this position was marked using a red waterproof marker to ensure later accurate positioning of the digital probe. Before each measurement the device was calibrated according to the manufacturer's instructions. All subjects were photographed in a standing upright position leaning against a wall with a retractor placed in their mouth. Subjects held a grey card under the upper front teeth when photographing, which serves as a color calibrator in digital image analysis. All photographs in this study were taken using a Smile Lite Mobile Dental Photography device. The Smile Lite MDP device was equipped with three different smartphones for photographing front teeth. Each time the smartphones in the device were replaced, the subjects had their retractor removed from their mouths to prevent dehydration. A total of 30 different photos in two formats (RAW and JPEG) were made for each participante, 15 for each photo format. All photos were imported to the computer program Adobe Photoshop Lightroom 6. All the photos were measured two times, once with and once without special calibration using gray card on the picture. For calibration white balance of each photo had to be carried out using the measurement probe placed on one of four gray segments on the card while adjusting image exposure balance by setting lightness (L*) at 75*, and a* and b* values at 0* on histogram. Then the measurement of CIE L*a*b* values on the tooth were conducted setting the measurement probe right in the middle of the red circle marked previously on each maxillary right central incisor. When measuring with no calibration the measurement of CIE L*a*b* values on the tooth were conducting immediately on the tooth, avoiding previous calibration of the grey scale. Results: Most CIE L*a*b* values on RAW and JPEG photographs on all three tested smartphones in different lighting conditions were statistically significantly different (p<0.05). The difference in most CIE L*a*b* color values between calibrated and uncalibrated photographs on all three smartphones was statistically significant (p<0.05). CIE L*a*b* values of calibrated and uncalibrated RAW and JPEG photos between all three tested smartphones differed statistically significantly (F=29.780;p= 0.000). The CIE L*a*b* values on uncalibrated RAW photos taken with an iPhone 11 Pro smartphone using a polarizing filter completely matched the same values measured by the spectrophotometer (p>0.05). Conclusions: Different lighting conditions, measurement procedures, and photo format in this study influenced the CIE L*a*b* values on smartphone photos. With the limitations of this research, it can be concluded that an uncalibrated RAW photo taken by an iPhone 11 Pro smartphone taken with a polarizing filter on the front LED light could be used in digitally determined tooth color under standardized conditions.