The maxilla and the mandible are richly supplied with blood vessels and nerves. In the presence of a certain dental pathology, with a detailed anamnesis, performed clinical examination and analyzed X-ray for the most accurate diagnosis and better treatment plan, we use additional diagnostic tests. Sensitivity tests are often misunderstood with vitality tests. Sensitivity tests examine the innervation of the dental pulp, and sensitivity tests examine its vascularization. A negative result on the sensitivity test means that the tooth is denervated, which does not necessarily mean that the tooth is avital. Vitality depends on its vascularization, which can be preserved even if the tooth is denervated, and it is examined by vitality tests. In this paper, the method of measuring the vitality of a laser Doppler flowmeter (LDF) is described in more detail. LDF is used to examine the vitality of the skin, oral mucosa, gingiva, alveolar bone, palatine mucosa and tooth pulp. By the difference between the frequencies of the outgoing and reflected rays, we can learn a lot about the vitality of the measured area, which greatly helps us to understand the pathology we encounter. Despite numerous researches and promising possibilities, it did not find wider clinical application due to its high cost, necessary additional education, signal interference with the surrounding tissue, need for better isolation of the examined area and less patient and device mobility, frequent calibration and extensive restorations that make measurement impossible.