prikaz prve stranice dokumenta Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu
  Download
PDF 2.09 MB
undergraduate thesis
Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu
Sisak: University of Zagreb, Faculty of Metallurgy, 2019. urn:nbn:hr:115:752253
Vuković, Anamarija
University of Zagreb
Faculty of Metallurgy

Institutional repository: Repository of Faculty of Metallurgy University of Zagreb

Cite this document

Vuković, A. (2019). Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu (Undergraduate thesis). Sisak: University of Zagreb, Faculty of Metallurgy. Retrieved from https://urn.nsk.hr/urn:nbn:hr:115:752253

Vuković, Anamarija. "Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu." Undergraduate thesis, University of Zagreb, Faculty of Metallurgy, 2019. https://urn.nsk.hr/urn:nbn:hr:115:752253

Vuković, Anamarija. "Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu." Undergraduate thesis, University of Zagreb, Faculty of Metallurgy, 2019. https://urn.nsk.hr/urn:nbn:hr:115:752253

Vuković, A. (2019). 'Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu', Undergraduate thesis, University of Zagreb, Faculty of Metallurgy, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:115:752253

Vuković A. Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu [Undergraduate thesis]. Sisak: University of Zagreb, Faculty of Metallurgy; 2019 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:115:752253

A. Vuković, "Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu", Undergraduate thesis, University of Zagreb, Faculty of Metallurgy, Sisak, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:115:752253

Metadata
TitleToplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu
Title (english)Thermal analysis of biocompatible materials for tissue engineering
AuthorAnamarija Vuković
MentorTamara Holjevac Grgurić (mentor)
Committee memberIvan Brnardić (predsjednik povjerenstva)
Committee memberTamara Holjevac Grgurić (član povjerenstva)
Committee memberStjepan Kožuh (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Metallurgy
Sisak
Defense date and country2019-10-10, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Metallurgy
Abstract
Tkivno inženjerstvo pruža velike mogućnosti u razvoju umjetnih organa, osobito bioloških regenerativnih materijala, kao što su hrskavica, kosti i koža. Dizajniranje odgovarajućih biorazgradljivih i biokompatibilnih materijala te praćenje biokemijskih i fizikalnih svojstava, kao i rasta stanica, omogućuje generiranje nadomjestaka koji mogu obnoviti ili znatno poboljšati funkciju oštećenih tkiva. U ovom radu, istražen je utjecaj različite topografije elektroispredenih polimernih nosača polikaprolaktona (PCL) na uspješnost zasijavanja i rasta stanica. Kolektori su dizajnirani programom 123D Design te su zatim 3D printani. Nosači polikaprolaktona i antibiotika Cefuroxima, CFU®, pripremljeni su elektroispredanjem, u koncentracijama antibiotika, 14 % PCL + 15 % mas. i 14 % PCL + 20 % mas.CFU®. Morfološka struktura pripremljenih nosača prije i nakon zasađivanja HeLa stanica, praćena je skenirajućom elektronskom mikroskopijom (SEM), a biofunkcionalnost nosača ispitana je MTT testom. Temperature kristalizacije (Tk) i taljenja (Tm) PCL/CFU® nosača te stupanj kristalnosti (c) određeni su diferencijalnom pretražnom kalorimetrijom (DSC), dok je toplinska stabilnost materijala praćena tehnikom termogravimetrije (TG). Dobiveni rezultati mikrostrukture nosača korelirani su s rezultatima dobivenih temperatura transformacija te koncentracijom antibiotika, kao i rasprostranjenošću i rastom stanica na nosaču.
Abstract (english)
Tissue engineering provides a great opportunities in the development of artificial organs, especially biological regenerative materials, such as cartilage, bone and skin. Designing the appropriate biodegradable and biocompatible materials and investigation of their biochemical and physical properties as well as cell growth factors, makes it possible to generate substitutes that can restore or substantially improve the function of damaged tissues. In this paper, the effect of different topographies of electrospun Polycaprolactone fibrous carrier on the incorporation and cell growth was investigated. Polymer carriers with different structure and porosity, have been prepared by electrospinning of PCL/CEFUROXIM® (CFU®) solution at the designed and 3D printed collectors. Polymer solution was prepared with different concentrations of antibiotic CFU®, 15 and 20 %wt. Fibrous morphology was followed by scanning electron microscopy (SEM), while biocompatibility was detected by MTT test. Melting (Tm) and crystallization (Tc) temperatures of PCL/CFU® as well as degree of crystallinity (c) were determined by differential scanning calorimetry (DSC), while thermal stability of material was obtained by thermogravimetry (TG). Results of PCL/CFU® carrier microstructure were correlated with transformation temperatures and antibiotic concentrations as well as cell growth.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:115:752253
ProjectNumber: IP-2016-06-6878
Title: Ciljana izrada prototipa vlaknastog nosača za uzgoj tkivnih stanica kombiniranim elektroispredanjem
Acronym: COMBOELECTROSPUN
Leader: Budimir Mijović
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Metallurgy; specializations in: Metallurgical Engineering, Industrial Ecology
Course: Industrial Ecology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka metalurgije (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka metalurgije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-10-14 07:03:41