The characterization of nonwoven agrotextile commercially available on the Croatian market, with the purpose of improving seed germination and the protection of perennial plants from frost, was conducted. Samples of polypropylene fibres were produced by a meltblown process, while samples of natural fibres (jute and coconut) are produced by mechanical process bonded with needle punching process. Mass per unit area of samples is in the range of 17 g m-2 to 980 g m-2. Basic physical-mechanical properties, i.e. mass per unit area, thickness, tensile properties, bursting strength as well as thermal and water vapor resistance were tested. In the previous research conducted 2016. and 2017., the influence of agrotextile on germination by planting seeds in the seeding cups and planting seeds directly in the soil of Zagreb County area, was conducted. The research has shown the positive influence of agrotextile on seed germination, i.e. using different masses per unit area of nonwoven agrotextile has enhanced the growth and development of seed plants from all seed cultures unlike to seeds that are not covered by nonwoven agrotextile. By increasing the mass of nonwoven agrotextile which covered the seeds, almost all kinds of sown seeds increase the height of plants. The largest plants height were recorded using nonwoven agrotextile of larger mass per unit area, i.e. 30 g m-2 and 40 g m-2. The height of plants grown on a field covered with nonwoven agrotextile of a mass of 40 gm-2 is up to 290% higher than the height of plants from the control field. In late autumn of 2017., the seeds of wintergreen lettuce were sown and covered with nonwoven agrotextile of three masses per unit area; 17 g m-2, 30 g m-2, and 40 g m-2. At spring of 2018., the height and weight of the lettuce were measured. By covering with nonwoven agrotextile mass per unit area of 40 g m-2, the highest salad heads grew up, while the highest yield in comparison to the control field yielded a field covered with nonwoven agrotextile of 17 g m-2 (83.3%), followed by a field covered with agrotextile of 30 g m-2 (34.7%) and finally nonwoven agrotextile of 40 g m-2 (29.2%). 8 By analysing plant material in winter salad it was found that the use of nonwoven agrotextile does not affect its quality, i.e. preliminary research suggests that the proportion of macroelements and microelements could be higher with respect to the wintergreen salad that was not covered. For a statistically reliable conclusion, it is necessary to make additional analyses of wintergreen salads through several consecutive harvests and chemical analysis of the soil. In the spring of 2018., the influence of different regions of Croatia on seed germination was conducted using the nonwoven agrotextile to improve commonly used for that purposes (18 g m-2) and agrotextile which had the best results in continental Croatia in the previous years (40 g m- 2). The impact of nonwoven agrotextile on the development and growth of seeds planted from seeds in the Continental and Coast area of Croatia was investigated with regard to the influence of climate and soil characteristics. Climate and soils determine the mass per unit area of nonwoven agrotextile that will improve seed germination. When selecting the mass of nonwoven agrotextile, it is necessary to take into consideration the soil and the forecast temperatures for the seed germination period in order to obtain the best possible germination results for a certain type of seed.