This work presents thin layer chromatographic determination of the content of alginate micro-capsules filled with silver nanoparticles that exhibit extremely strong antimicrobial and antiba-cterial properties, which are suitable for application on textile materials and their surface fun-ctionalization. Silver nanoparticles have anti-bacterial effects on a wide range of Gram-positive and Gram-negative bacteria, including antibiotic-resistant strains. They are widely used beca-use of their chemical stability, catalytic activity, and high conductivity, as well as their excepti-onal antimicrobial activity due to their high surface-to-volume ratio, which provides them with better contact with microorganisms. Encapsulation methods are used in the textile, pharmace-utical and cosmetic industries to deliver biologically active substances to desired places. The availability of new active nanoparticles has opened up opportunities for the development of new matrices and applications that can be used in the development of various products, and encapsulation is a relatively new technology that enables the storage, stability and slow rele-ase of active substances. Materials for forming microcapsules are mainly carbohydrates (starch, cellulose, gums), proteins (milk proteins - caseins and whey proteins, gluten, gelatin) and lipids (fatty acid-line, alcohols, glycerides, waxes, phospholipids) or a combination thereof. Microcapsules are becoming increasingly present in the functionalization of textile polymers. Techniques for encapsulating bioactive constituents include co-conservation, micro-encapsu-lation by hot-air flow, micro-encapsulation of fluidized particles, micro-encapsulation by extru-sion and supercritical fluids, emulsification and cocrystallization. In this work, microcapsules were investigated by thin layer chromatography to determine the silver content of the samples. The chromatographic method has proven to be a very fast, efficient and cost-effective method advantageous over many other instrumental methods and can be threfore recommended for usage in many test laboratories.