The teaching of Science class, as a complex structured and interdisciplinary subject, for quality teaching requires professionally and methodologically qualified persons, that is teachers. The qualification of teachers is manifested through the acquired competencies, important for the subject, in the methodological courses Methodology of Science. The aim of this research was to determine the ability of fifth year students of teacher study to independently teach Science class. 63 students participated in the online research (60 from the University of Zagreb, 2 from the University of Osijek and 1 from the University of Rijeka). The task of the participants was to determine agreement with the statements related to the theoretical part of the methodology, the practical part of the methodology, analysis of methodical exercises and opinion on the readiness and ability to work independently in the teaching process of Science class. The results show that the participants of the University of Zagreb assess that they are qualified for independent work after passing the course Methodology of Science. Then, a statistically significant difference was found in the opinion of students of the departments in Zagreb and Petrinja and students of the department in Čakovec regarding the adoption of important theoretical content for methodological exercises, perception of the importance of analysis for training students and training skills after graduation. With the independence of writing the preparation, there is no statistically significant difference between the departments. The analysis of the results of the participants from the University of Osijek and Rijeka shows high results as well as the participants from the University of Zagreb. Based on the obtained results, it can be concluded that the courses Methodology of Science are a well-structured courses because they qualitatively prepare students for further work independently of the University.