Physical activity has an irreplaceable value because it is the best mean to achieve and preserve human health and therefore it is important to include children of early and preschool age in physical exercise and the preschool teacher has an extremely important role in that process. The theoretical part explains the importance of physical exercise for children of early and preschool age, the developmental characteristics of preschool children are mentioned as well. Types of physical exercise of children in kindergarten are stated, the material environment for conducting physical exercise described and the role of preschool teacher in conducting physical activities with children of early and preschool age. In the research 98 preschool teachers were surveyed with the aim of identifying the challenges that preschool teacher face while conducting physical activities. The results show that most respondents are aware of the importance of performing physical activities, but only a third of them perform physical activities three or more times a week. It was also noticed that some kindergartens do not have gyms or adequate space to conduct physical activities. Increased number children in groups make it difficult for preschool teachers to assist the children and ensure that all the exercises are conducted in a proper and safe manner. Preschool teachers believe that condition of kinesiological activities have the same value as other activities in the educational process.