The aim of this research was to determine how does the physiological load move in the kinesiology class with preschool children. One hour of Kinesiology class was spent with preschool children in the kindergarten „Iskrica“ where student exercises are held. Accordingly, the children are very well acquainted with the way of working, which resulted in a successful and well-conducted class. The alternate-departmental methodological-organizational form of work was used and the lesson lasted 35 minutes. The research sample included 11 children of which 6 boys and 5 girls. In order to determine how the physiological load moves two measures were used to measure heart rate. The „Heart Rate Monitor“ application was used for measurement. Heart rates were measured before the start of the class and after each subsequent part of the class. The obtained data were structured in a table and processed in program Statistica 13. By processing the data we got the obtained curves of physiological load movement during the whole Kinesiological class. After an initial measurement of 85 heartbeats, the heart rate increased in the introductory part of the class and peaked in the general preparatory part when it was 110. This was followed by a decrease in heart rate after the first change in the main „A“ part of the class. However, after the next changes, the pulse increased significantly, only to fall again in the third shift and continue to fall until the very end of the class. The obtained results do not coincide with the ideal scheme of physiological load of E. Vukotić, who states that the physiological load should gradually increase during class and in the main „B“ part return to approximately the same values that were at the beginning of the class. Possible causes of such results are inappropriate content, wrong choice of methodological and organizational form of work or insufficient experience of educators. Also, a comparison with previous researches shows discrepancies, but also some similarities between the obtained results and the reasons why they occurred.