Vaccination is one of the most efficient preventive methods of fighting disease and is one of the biggest causes of many dangerous diseases being rooted out. The point of vaccination is not only the protection of the individual, but also the entire community, which is why a member of the community contracting such a disease poses a threat not only to the individual but also to everyone around them. The Covid-19 pandemic has shaken up the world in many ways and caused deep divisions in our society. For a full year the world was in the grips of fear and people eagerly awaited a vaccine that would eradicate the disease, but when the vaccine was finished doubt started to crawl into people and all kinds of conspiracy theories started to take form further dividing the already divided society. This paper consists of two sections, the theoretical and the research section. In the theoretical section the reader is introduced to the topics vital for the easier understanding of the research section of the paper which deals with the problems which arise from the topics discussed in the theoretical section. In the theoretical section the following is listed and explained: mandatory vaccinations, legislation regulating vaccination of children, the creation of the first vaccine, the beginnings of the anti-vaccination movement, the Covid-19 pandemic in Croatia and the development of the Covid-19 vaccine. The aim of this paper is to assess the attitudes that parents/guardians of preschool children have towards vaccination, as well as whether this pandemic changed their views on vaccination of children and, if yes, in which ways. This was done through a questionnaire designed for this research paper and the results were then analyzed. The results showed that the majority of parents trust the mandatory vaccinations, but still maintain a dose of caution. When compared to research carried out three years ago, the results show a double increase in the number of those who think that vaccination of children should not be mandatory. The pandemic and the anti-vaccination movements have left their marks on the parents’ minds and have shaken up their attitudes.