Educational inclusion is vital in today's modern world. Since there are more and more children with disabilities in today's society, educational inclusion is inevitable in institutions of early and preschool education. On daily basis educators are facing increasing challenges in their educational work with children of early and preschool age. Therefore, lifelong learning, development and strengthening of theirs professional competences are extremly important for inclusive practice and cooperation In order to ensure well-being and optimal development of every child in an inclusive kindergarten, it is necessary to accomplish a collaborative relationship between educators and parents. In an inclusive kindergarten all participants of educational process should be open and ready for collaboration and teamwork. By sharing their own experiences and proffessional knowledge, educators and associates contribute to optimal conditions for the growth and development of every child, especially children with disabilities. Inclusive education is aimed at strenghtening cooperation and active participation, therfore this paper examines educators attitudes and opinions about cooperation and teamwork in an inclusive kindergarten. The aim of this research is to examine educators attitudes and opinions regarding collaboration and teamwork with parents, professional associates and assistants working with children with developmental disabilities. Research was conducted with an online questionnaire “Collaboration and teamwork in inclusive kindergarten, which was created for this thesis. The results showed that educators are mostly satisfied with collaboration with parents and assistants for children with developmental disabilities, while they are less satisfied with collaboration and teamwork with professional associates. The conducted research shows that good and high-quality collaboration with parents, professional associates and assistants for children with developmental disabilities is very important for educators. It contributes not only greater satisfaction in educational work for educators, but also contributes for better environment in kindergarten.