The aim of this paper is to examine the metric characteristic of a measuring instrument for measuring the motor skills of preschool children and to investigate whether there is a significant difference between first, second and third measuring of each of the motor skills. Kinesiological activity is an important aspect of every person's life, especially a child's because it grows and develops. Childhood is the key period for influencing on development of motor skills but also the adoption of healthy habits that will remain with the child for life. Motor skills are one of the most important areas of child's development and are often linked to the develompent of other areas, such as speech. The research was conducted on 98 children between the ages of 4 and 6, of which 51 children attend regular program of physical education in kindergarten, while the other 47 attend a sports program in addition to the regular program in kindergarten. The measurements were carried out in three kindergartens in the city of Zagreb, in the spring of 2020. For the purpose of this research, four motor abilities, speed, strength, flexibility and balance, were measured using four tests, running on all four around cones (KRČ), standing long jump (SDM), sitting forward bend (PRS) and standing on one leg (SJN). Each test was performed three times. Based on the results of the metric characteristic, it can be concluded that the performed tests are suitable for children age 4 to 6 and that they are sufficiently sensitive, reliable and homogenic, while the correlation between particles, obtained by Inter-Item correlation, is very high.