Background: Shift work is increasingly becoming a widespread form of work organization in many healthcare systems, but it brings with it specific challenges for the health and well-being of workers. Sleep disorders, as one of the most common problems associated with shift work, can have a significant impact on the general state of health and quality of life of healthcare workers. This research focuses on investigating the impact of shift work on sleep disorders, health, physical activity and diet of respondents. Aim: The objective of the research is to examine the connection between shift work and sleep disorders of hospital nurses and technicians. Subjects and methods: A total of 96 respondents of different genders and age groups participated in the research. Respondents were divided according to gender, age, level of education and work experience in shifts. The data was collected through an anonymous questionnaire that included questions about the type of shift work, the impact on sleep, general health, physical activity, eating habits, and the experience of symptoms of sleep disorders. Results: The analysis of the obtained data revealed that most respondents (74%) state that shift work affects their sleep, with more frequent symptoms of insomnia and nightmares. Also, most respondents (83.3%) feel that shift work affects their general state of health, with an emphasis on metabolic disorders and mental health. As for physical activity and eating habits, 66.7% of respondents notice a negative impact of shift work on their physical condition and diet. Conclusion: These results clearly show that shift work has a significant impact on the health and well-being of nurses and technicians, especially regarding sleep disorders, general health, and physical activity and dietary habits. Interventions aimed at improving the conditions of shift work and supporting nurses in adapting their lifestyle can be crucial in reducing the negative effects of this work on the workforce.