In the contemporary world, a growing emphasis is placed on the importance of professional occupation as a source of meaning, purpose and fulfillment. Many spend an increasing amount of time at work, defining themselves through what they do, which means that one is satisfied when their job contributes to his/her personal identity. Job satisfaction stems from the individual's perception, which evaluates the suitability of the job to their personal identity. Additionally, job satisfaction is linked to the employees’ well-being observed through two approaches: hedonistic well-being, focused on subjective experiences of enjoyment and life satisfaction, and eudaiomistic well-being, focused on purpose and self-realization. In various studies, both approaches are integrated into the construct of psychological flourishing. In previous research which studied well-being and job satisfaction the traditional job characteristics (payment, management, working conditions and similar) were analysed. In recent studies, an increasing emphasis is put on understanding the subjective experience of professional occupation which includes work significance, work enthusiasm and work inclination, the characteristic also analysed in this study. Sixty-two higher education professors participated in the study. The aim of this paper is to examine how work significance, work enthusiasm and work inclination contribute to one’s well-being and job satisfaction. The carried out regression analyses show that work enthusiasm is a positive predictor of job satisfaction, followed by work significance as a positive predictor of psychological flourishing, while work inclination on a job as a source of income is a negative predictor for both job and life satisfaction.