Introduction: Psychosis is a frequent mental disorder which entails an impaired capacity of testing reality. Its defining features are the presence of hallucinations and/or delusions. A disease is a cause of a substantial subjective suffering, both for affected as well as for their families. It also aggravates functioning in different spheres of the everyday life. The treatment approach is multidimensional and to a large extent it is based on specific factors causing psychosis. The factors causing psychosis are multiple and include various biologic and environmental factors, while the interaction of different factors is particularly emphasized. A better understanding of an interaction of genetic and various environmental factors can contribute to better prevention amid an indentification of risk groups, as well as to devise a more successful multidimensional treatment approach. Objective: The aim of this study was to establish the presence of the association between genetic and envireonmental factors among those diagnosed with acute psychosis. Methods: We carried out a retrospective observational investigation on 120 patients treated at the Department for the first-episode psychosis, at the Vrapče University Psychiatric Hospital, in the period between 2015 and 2020. The data is collected from from the existing medical documentation and the electronic database of the Institute for the first-episode psychosis. Results: We established statistically significant association between parental separation and the use of psychoactive substances among diagnosed with acute psychosis. At the same time, we do not find statistically significant association between the psychiatric heredity and two environmental factors. Conclusion: Environmental factors can act as independent factors in causing acute psychosis. The statistically significant association between parental separation and the use of psychoactive substances conveys risk groups for developing psychosis and points to the importance of proactive and multidimensional approach for reducing psychiatric morbidity.