In recent decades, medical tourism has proved to be an excellent bait for tourists taking into account the quality of the service but also the price. Because of its exceptional geographic location and its connection with the rest of the country (and the world), Northwestern Croatia is gradually becoming the center of medical tourism in the region. The graduate thesis "Public Relations in the function of the development of medical tourism - example of Hotel Istra in Varaždin" explains the more detailed topic of medical tourism and that the development of globalization and technology in the 21st century brought about progress in the field of public relations, which further enabled the stronger connection of communities, exchange of information, intensified communication and overall connectivity. In such environment, public relations are of great importance for further development of medical tourism. In this graduate thesis, the focus is placed on medical tourism and the hotel '' Istra ''. It defined the concept of public relations in practice, analyzed the process and activities of public relations with regard to marketing, described the main characteristics and characteristics of medical tourism, analyzed the strategic plan for the development of medical tourism on the example of Hotel Istra, the state of medical tourism in the Republic Croatia with a review of the competition, set a mission and vision, conducted a SWOT analysis and considered the marketing environment, defined the strategy and manner of public relations, consumer behavior, and defined consumer groups. We ended up with a survey of "People'sWays to Hotel and Medical Tourism" to find out the habits and the interest of people, then came to a conclution.