The process of transferring blood or a blood product from one person's bloodstream to another person's bloodstream is called a transfusion. Transfusion treatment is a specific form of treatment. The way in which it is carried out is a clinical assessment and the very usefulness of transfusion treatment due to the great possibility of side effects. Donated whole blood can be processed into red blood cells, platelets, fresh frozen plasma and cryoprecipitate. This processing allows the administration of individual blood components to several different patients and the transfusion of appropriate blood components according to the specific needs of the individual. One of the key and successful transfusion treatments is the nurse. Their role begins with the very identification of the patient and ends with the administration of blood products and recognition of transfusion reactions. The safety of transfusion treatment is greatly influenced by the procedures of nurses/technicians. Although blood transfusion may be considered a common practice, it carries with it a high risk. Healthcare personnel should be aware of their roles and responsibilities in the transfusion process. In order to reduce the risk factors associated with transfusion, continuous education is needed. The aim of the research was to examine the knowledge and attitudes of nurses/technicians about transfusion treatment and to determine the presence of a statistically significant difference in attitudes and knowledge with regard to gender and professional education. The research was designed as a cross-sectional study. 172 nurses/technicians from northwestern Croatia participated in the research. The research was conducted in the second half of July and the beginning of August 2022. Two hypotheses were presented. The results showed that medical technicians have worse attitudes about transfusion treatment than nurses, but the difference is not statistically significant. The statistical analysis showed that the medical staff of VSS have a higher level of knowledge than the employees of lower professional programs. Also, the difference in knowledge is not statistically significant either. In the Republic of Croatia, the day of voluntary blood donors is celebrated every year on October 25.