Title Usporedba mikrobiloških testova u dijagnostici COVID - 19 bolesti Title (english) Comparison of microbilogical tests in the diagnosis of COVID - 19 disease Author Sanja Antić Mentor Lorena Lazarić Stefanović (mentor)Committee member Emina Pustijanac (predsjednik povjerenstva)Committee member Lorena Lazarić Stefanović (član povjerenstva)Committee member Nada Tadić (član povjerenstva) Granter University of Pula Defense date and country 2021-09-29, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Pod pojmom COVID-19 podrazumijevamo akutnu respiratornu bolest uzrokovanu SARS-CoV-2 virusom koji ima visoku stopu infektivnosti. Higijena ruku, površina, fizička distanca i korištenje maski, predstavljaju optimalan način sprječavanja prijenosa bolesti. Ljudi zaraženi virusom SARS-CoV-2 mogu razviti različite simptome i u težim slučajevima mogu dovesti do smrti, ali bolesnici mogu biti i asimptomatični. Radi brzog širenja bolesti, pojavila se potreba za brzom i pouzdanom dijagnostikom. Primarne dijagnostičke metode za COVID-19 su RT-qPCR (reverzna transkriptaza kvantitativne lančane reakcije transkriptaze) i BAT (brzi antigenski test) testovi. RT-qPCR testovi izvode se u zdravstvenim ustanovama opremljenim automatiziranom opremom i vrlo su pouzdani. BAT testovi testiraju se na licu mjesta, vrlo su jednostavni za uporabu, pogodni i pouzdani za brzo otkrivanje infekcije i tako učinkovitiju kontrolu pandemije, a imaju usporedivu točnost i osjetljivost u usporedbi s RT-qPCR testovima. Cilj rada bio je prikupiti i analizirati podatke o ispitanicima koji su bili testirani na COVID - 19 bolest, te usporediti ispitanike koji su bili testirani BAT i RT-qPCR testom istih uzoraka u populaciji. Prikazane podatke koristili smo iz Opće bolnice Pula po dobivenoj suglasnosti Etičkog povjerenstva. U razdoblju od 11.12.2020. do 05.02.2021. rezultatima istraživanja zabilježeno je 298 pacijenata koji su testirani BAT testom, ali zbog sigurnosti, njih 141 je napravilo i RT-qPCR test. Usporedbom oba testa zaključujemo da se najčešće koristio BAT-test zbog jednostavne i brže dijagnostike, a PCR metoda kada je za to bila jasna indikacija a BAT test nalaz negativan.
Abstract (english) Under the term COVID-19 we mean acute respiratory disease caused by SARS-CoV-2 virus with a high infectivity rate. Hand hygiene, surfaces, physical distance and use of masks are the optimal way of preventing the coronary disease. People infected with SARS-CoV-2 may develop different symptoms and may lead to death in more severe cases, but patients may also be asymptomatic. In order to rapidly spread the disease, there is a need for quick and reliable diagnostics. The primary diagnostic methods for COVID-19 are RT-qPCR (Rever-know transcriptase of qu-antitative chain reactions of transcriptase) and BAT (rapid antigen test) tests. RT-qPCR tests are performed in health facilities equipped with automated equipment and are very reliable. BAT tests are tested on the spot, they are very simple for use, suitable and reliable for quick detection of infection and thus more efficient control of pandemic, and they have comparable accuracy and sensibility compa-red to RT-qPCR tests. The aim of this study was to collect and analyse floor data on subjects who were tested for COVID - 19 disease, and to compare subjects who were tested for BAT and RT-qPCR Tes-th of the same samples in the popula-tion. We used the presented data from the Pula General hospital according to the approval of the ethical nation In the period from 11.12.20. to 05.02.2021., 298 pa-tients were tested with a BAT test, but for safety reasons, 141 of them were done and a RT-qPCR test. By comparing both tests, we conclude that the BAT test was most frequently crushed because of simple and faster diagnostics, and the PCR method when it was clearly indicated and the BAT test was negative.
Keywords
mikrobiologija s parazitologijom
COVID-19
SARS-CoV-2 virus
RT-qPCR test
BAT-test
negativna prediktivna vrijednost
Keywords (english)
Microbiology with parasitology
COVID -19
SARS-CoV-2 virus
RT-qPCR test
BAT-test
negative pre-dictive value
Language croatian URN:NBN urn:nbn:hr:137:993261 Study programme Title: Nursing Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2021-10-04 13:48:03
