This master's thesis deals with the trade relations between the Republic of Venice and the Ottoman Empire during the 16th and 17th centuries in Dalmatia. The first chapter describes the history of Dalmatia through this period, with special emphasis put on the trade and the negative impact of wars and other problems, such as diseases and thefts. In the next chapter, an account of the trade of the Bosnian Pashalik is given, i.e., how the trade developed, and which cities were the most important trade centres. Likewise, the role of the Republic of Dubrovnik in the trade between the interior of the Balkans and Western Europe is described and how Dubrovnik took advantage of the period of the Ottoman-Venetian wars. What is more, the main characteristics of the trade are explained, and its most important products are listed. The last chapter deals with the trading of the coastal ports of Gabela on the Neretva river, Makarska, Šibenik, Zadar, Obrovac and Skradin, i.e., it is illustrated how the trade between the Venetians and the Ottomans took place through these cities. In the end, the importance of the port of Split, which stood out as the most prominent port on the trade route between the Ottoman Empire and Western Europe, was described.