This thesis deals with describing different phenomenom connected to cultural heritage. Primarely it discusses different definitions of the concept as well as the types of legal and financial aspects of protection of that sort of material. The process of digitalization as a form of preservation and presentation of cultural heritage is also shown. Criteria for selection of material and technology, along with the explanation of some problems such as sustainability and financing of digital collections. A comparison of national and international guidelines for digitalization of cultural heritage is given, as well as of metadata description standards for digital collections, and examples of national and international digitalization initiatives and projects, for example Europeana (a collection of millions of digitalized materials, along with music and video content from various European museums, libraries, archives and collections in general). Speaking of national collections, the thesis is also presenting national programme of digitalization of archival, library and museum materials, which is conducted through several different projects: Hrvatska kulturna baština, Digitalizirana zagrebačka baština by The Zagreb City Libraries and Zavičajna zbirka Spalatina, which has been conducted since 2009 by the Marko Marulić City Library from Split. The research part of the thesis is about Zavičajna zbirka Spalatina (Spalatina Regional Collection) and Digitalizirana zagrebačka baština (Digitized Zagreb Heritage), in a way that a historical overview is given, interfaces between two libraries are compared and described, as well as certain elements of their collections, such as type and number of objects held in them or which elements for a metadata description are involved.