Wax, a product of the wax glands of 12 to 18-days-old honey bee workers, is a building block of the comb. The workers store food (nectar, honey, pollen) in the combs, and the queen bee stores the eggs from which the bees’ nest is developed. The specific chemical structure of wax enables the deposition of harmful compounds from the environment, including heavy metals. Given that every member of the honeybee colony, the nest and all the honeybee products come into contact with the wax, the harmful compounds contained in it can threaten the functioning and health of the entire colony, and indirectly, through the honeybee products, the health of human consumers. As the wax (honeycomb) ages, its quality decreases, so it necessity to be processed in order to be purified. Moreover, it must be sterilized to prevent the spread of dangerous diseases. During the wax processing, using the method of extended cooling and sedimentation, the wax samples were taken from different levels (layers) of the tank. We determined the concentrations of arsenic, cadmium, lead and mercury in the samples using inductively coupled plasma - mass spectrometry (ICP-MS). The mercury levels were tested on a mercury analyzer and compared the results with the concentrations allowed according to European legislation. Of all the metals and wax layers, only the concentration of lead exceeded the permissible concentration of 5 mg/kg in S4, and peaked at 13.39 mg/kg. Given that this wax layer is discarded before the new sedimentation and cooling phases, there is no danger of an impermissible concentration of lead in the processed wax or in the new comb foundations. Therefore, we can consider this method effective and desirable for reducing the concentration of heavy metals in processed wax.