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master's thesis
Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2023. urn:nbn:hr:178:529475
Unger, Mislav
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Unger, M. (2023). Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:529475

Unger, Mislav. "Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:178:529475

Unger, Mislav. "Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:178:529475

Unger, M. (2023). 'Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:529475

Unger M. Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2023 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:178:529475

M. Unger, "Uporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:178:529475

Metadata
TitleUporaba mikroskopskih i molekularnih metoda u dijagnostici dermatofitoza u zamorčića
Title (english)The use of microscopic and molecular methods in the diagnosis of dermatophytosis in guinea pigs
AuthorMislav Unger
MentorSuzana Hađina (mentor)
Committee memberMatko Perharić (predsjednik povjerenstva)
Committee memberZrinka Štritof (član povjerenstva)
Committee memberSuzana Hađina (član povjerenstva)
Committee memberVilim Starešina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2023-05, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Dermatofiti su keratinolitičke gljivice koje mogu prouzročiti kožne promjene u životinja i ljudi. Zamorčići su česti asimptomatski nositelji dermatofita, što je važno imajući u vidu njihovu sve veću popularnost kao kućnih ljubimaca. Rad mikoloških dijagnostičkih laboratorija koristi klasične metode laboratorijske identifikacije koje uključuju izravnu mikroskopsku pretragu dlake, izdvajanje dermatofita na hranjivoj podlozi te njihovu identifikaciju na osnovu makroskopskih i mikroskopskih obilježja kolonija. Uporaba molekularnih metoda pokazala je da je neke vrste dermatofita teško razlikovati međusobno samo na osnovu morfologije, ali i moguće zamijeniti s nekim vrstama saprofitskih plijesni. Cilj ovog istraživanja bio je utvrditi prisutnost dermatofita na koži i dlaci zamorčića koristeći izravno mikroskopiranje dlake, kulturelnu pretragu te identifikaciju vrsta dermatofita na osnovu makroskopskog izgleda kolonija i mikroskopskih obilježja gljivičnih elemenata, lančanom reakcijom polimerazom (engl. polymerase chain reaction, PCR) i sekvenciranjem. Od sveukupno 24 uzoraka dlačnog pokrivača podrijetlom od zamorčića kulturelnom pretragom, lančanom reakcijom polimerazom te sekvenciranjem iz njih četiri izdvojena su i identificirana tri dermatofita vrste Trichophyton benhamiae i jedan vrste Nannizzia gypsea. Izravnom mikroskopskom pretragom nije utvrđena invazija dlaka artrosporama dermatofita. Kulturelna pretraga je omogućila preciznu identifikaciju dermatofita, ali nije bila dostatna za preciznu identifikaciju u slučaju tri narasle kulture plijesni sličnih dermatofitima koje su identificirane PCR metodom i sekvenciranjem kao vrste Chrysosporium lobatum, Chrysosporium minutisporosum i Aphanoascus fulvescens. Prevalencija emergentnog patogenog dermatofita Trichophyton benhamiae na dlačnom pokrivaču zamorčića ukazuje na potrebu pojačane kontrole zdravlja zamorčića bez kliničkih znakova dermatofitoze, a u svrhu sprječavanja širenja ove zoonoze.
Abstract (english)
Dermatophytes are keratinophilic fungi that can cause skin lesions in animals and humans. Guinea pigs are common asymptomatic carriers of dermatophytes, which is important considering their increasing popularity as pets. Mycological diagnostic laboratories use classical methods of laboratory identification, including direct microscopic examination of hairs, isolation of dermatophytes on culture media, and their identification based on macroscopic and microscopic characteristics of colonies. The use of molecular methods has shown that some dermatophyte species are difficult to distinguish from each other based on morphology alone and can also be confused with some saprophytic mold species. The aim of this study was to determine the presence of dermatophytes on skin and hair of guinea pigs by direct microscopic examination of hair, culture testing, identification of dermatophyte species based on macroscopic appearance of colonies and microscopic characteristics of fungal elements, polymerase chain reaction (PCR) and sequencing. From a total of 24 guinea pig hair samples, four were isolated by culture test, polymerase chain reaction and sequencing, and identified as dermatophytes: three of them as Trichophyton benhamiae and one species of Nannizzia gypsea. Direct microscopic examination did not reveal invasion of the hairs by dermatophyte arthrospores. Cultural examination allowed accurate identification of dermatophytes but was not sufficient for accurate identification in the case of three cultures of molds resembling dermatophytes. These were identified by PCR and sequenced as species of Chrysosporium lobatum, Chrysosporium minutisporosum and Aphanoascus fulvescens. The prevalence of the emerging pathogenic dermatophyte Trichophyton benhamiae on guinea pig hair indicates that increased health monitoring of guinea pigs without clinical signs of dermatophytosis is needed to prevent the spread of this zoonosis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:529475
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-06-16 09:33:43