prikaz prve stranice dokumenta Ovce kao sentinel životinje za bolest Zapadnog Nila
  Download
PDF 503.1 KB
master's thesis
Ovce kao sentinel životinje za bolest Zapadnog Nila
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2023. urn:nbn:hr:178:809202
Mašović, Viktor
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

Cite this document

Mašović, V. (2023). Ovce kao sentinel životinje za bolest Zapadnog Nila (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:809202

Mašović, Viktor. "Ovce kao sentinel životinje za bolest Zapadnog Nila." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:178:809202

Mašović, Viktor. "Ovce kao sentinel životinje za bolest Zapadnog Nila." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:178:809202

Mašović, V. (2023). 'Ovce kao sentinel životinje za bolest Zapadnog Nila', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:178:809202

Mašović V. Ovce kao sentinel životinje za bolest Zapadnog Nila [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2023 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:178:809202

V. Mašović, "Ovce kao sentinel životinje za bolest Zapadnog Nila", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:178:809202

Metadata
TitleOvce kao sentinel životinje za bolest Zapadnog Nila
Title (english)Sheep as sentinel animal for West Nile disease
AuthorViktor Mašović
MentorLjubo Barbić (mentor)
MentorMaja Maurić Maljković (mentor)
Committee memberVilim Starešina (predsjednik povjerenstva)
Committee memberNevenka Rudan (član povjerenstva)
Committee memberMaja Maurić Maljković (član povjerenstva)
Committee memberLjubo Barbić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2023-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Virus Zapadnog Nila (WNV) je emergentni zoonotski uzročnik koji na području RH posljednjih desetak godina uzrokuje oboljenja ljudi od kojih su neka i sa smrtnim ishodom. Zbog toga se kontinuirano provodi sustav nadzora virusne aktivnosti korištenjem konja i peradi kao sentinel životinja. Kako bi istražili mogućnost unaprjeđenja sustava nadzora, u ovom radu smo istražili mogućnost korištenja ovaca kao sentinel životinja. U tu svrhu 300 seruma ovaca s područja Vukovarsko-rijemske županije pretražili smo imunoenzimnim testom (ELISA) te sve s pozitivnim ili sumnjivim rezultatom dodatno potvrdnim virus neutralizacijskim testom (VN-testom) uz korištenje tri flavivirusa koja cirkuliraju na području RH. Rezultati su pokazali da je ELISA test nedostatno specifičan za rutinsku dijagnostiku, a VN-testom su potvrđene infekcije ovaca WNV, virusom krpeljnog encefalitisa (TBEV) i po prvi put uopće infekcije ovaca usutu virusom (USUV). Potvrđena je kocirkulacija sva tri virusa na istom prostoru uz najvišu seroprevalenciju infekcija TBEV koja je sveukupno iznosila 9,7 % uz razlike po lokacijama od 5,0 % do 15,0 %. Ovaj rezultat dodatno zabrinjava jer ovce mogu TBEV izlučivati mlijekom zbog kojega može doći do oboljenja ljudi. Seroprevalencija WNV je 3,0%, a najniža seroprevalencije je bila USUV (1,3 %). Uz to potvrđene su i križne reakcije ili moguće istovremene infekcije različitim flavivirusima u 1,3 % životinja. Analizirani su i čimbenici rizika te je dokazano da je udaljenost mjesta držanja ovaca od kućanstva proporcionalna povećanju vjerojatnosti od infekcije TBEV. Sveukupno istraživanjem je potvrđeno da ovce mogu biti sentinel životinje za flavivirusne infekcije te da se pretraživanjem ovaca VN-testom kao metodom izbora, može nadzirati prisustvo i aktivnost WNV, TBEV, ali i USUV što je dokazano po prvi put.
Abstract (english)
In the last decade, the West Nile virus (WNV) is an emerging zoonotic disease in Croatia, causing illness and fatalities in people. The surveillance of viral activity is continuously implemented using horses and poultry as sentinel animals. This study aimed to investigate sheep as the possible sentinel animals in the surveillance program of WNV. The study was conducted on sheep sera from Vukovarsko-srijemska County in May 2022. In total, 300 sheep sera were tested using the ELISA test. Additionally, all positive and suspicious ELISA results were examined with VN-test using all three flaviviruses circulating in Croatia. The results revealed that ELISA is insufficiently specific for routine diagnostics. On the other hand, VN-test confirmed cases of WNV, TBEV and, for the first time, USUV infection in sheep. TBEV had the highest seroprevalence (9.7 %), ranging from 5.0 % - 15.0 % between locations. This result is concerning because TBEV can be found in sheep milk, which can be a source of human infection. WNV seroprevalence was 3.0 %, and USUV had the lowest seroprevalence (1.3 %). In addition, cross-reactions or possible simultaneous infections with different flaviviruses were confirmed in 1.3 % of animals. Risk factors analysis revealed that the distance of sheep habitat from houses is proportional to the probability of infection with TBEV. Overall, this research confirmed that sheep could be used as sentinel animals in flavivirus surveillance programs. They could detect the presence and activity of WNV, TBEV, and USUV with a VN-test as a diagnostic method of choice.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:809202
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-09-12 11:28:50