Ticks are obligate, hematophagous ectoparasites which are vectors of various infectious and parasitic animal diseases and zoonoes (viruses, bacteria, protists and helminths). Only specific species of hard ticks are involved in disease transmission, and their distribution greatly depends on the season and geographical location. The aim of this research was to identify the species, gender and developmental stage of ticks and to determine the prevalence of hard ticks in Osijek-Baranja County considering the study area and the months of the year. Using the obtained results, areas of potential risks of infection with various tick-borne diseases agents have been established. Ticks were sampled from the environment using the „dragging“ and „flagging“ method, and their developmental stage and species were identified using existing keys. The determination results are presented with original sketches and photographs, while the prevalence is shown using tables and graphs. The species sampled were Ixodes ricinus, Ixodes hexagonus, Haemaphysalis inermis, Dermacentor reticulatus, Dermacentor marginatus and Rhipicephalus sanguineus. Among all species, I. ricinus is the most abundant with a prevalence of 43,51% (57/131). R. sanguineus constitutes 31,29% (41/131) of the total number of sampled ticks and was found only within the city of Osijek, while H. inermis was found solely on Gajić, accounting for 16,79% (22/131). During the research, D. reticulatus ticks were found with a prevalence of 6,87% (9/131) in the village of Kopačevo, as well as one D. marginatus female tick in the vicinity of Lug. One I. hexagonus nymph was sampled in the vicinity of Josipovac. In conclusion, based on this research, the species posing the highest risk of transmission of tick borne diseases due to its distribution in the Osijek-Baranja County is I. ricinus. However, the vector potential of H. inermis to the north on Gajić and R. sanguineus in the city of Osijek should not be ignored.