Introduction: Vehicles and drivers are one of the most significant biosecurity risks for the transmission of disease agents within pig production units. According to European Union regulations, all pig transport vehicles must be cleaned and disinfected each time after unloading, and when necessary before each loading of animals, using disinfectants approved by the competent authority. This specialist thesis aimed to determine the effectiveness of sanitary washing and disinfection using two commercially available disinfectants on pig transport vehicles. The assumption was that the total number of bacteria after washing and disinfection would be reduced by about 90%, and more effective disinfectant will be recommended in the company's future practice. The main goal was to create its own standard operational protocol for the cleaning and disinfection of vehicles for the transport of pigs. Material and methods: Implementation control of sanitary washing and disinfection of vehicles for the transport of pigs was carried out during November and December 2022 by taking swabs from seven positions inside the four trucks, after unloading the pigs before washing, after washing and after disinfection, on five occasions. Two trucks were disinfected with a commercially available disinfectant based on an oxidizing agent, and two with a disinfectant whose main active ingredient is glutaraldehyde. The swabs were analyzed in the laboratory for the total number of bacteria and the number of enterobacteria. The results were processed statistically. Results and conclusions: The research confirmed the assumption because washing reduced the total number of bacteria and enterobacteria by more than 90% in all positions. The remaining total number of bacteria was significantly reduced after disinfection, ranging from 33 to 100%, for both used disinfectants. The number of enterobacteria significantly decreased in all positions after disinfection, and disinfectant based on an oxidizing agent proved to be more effective, especially in positions where larger amounts of organic matter are usually found. In order to make it easier to carry out and control the application of the procedures for washing and disinfection of trucks for the transport of pigs, a proposal of our standard operating protocol was made.