prikaz prve stranice dokumenta Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi
Access restricted to students and staff of home institution
master's thesis
Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2015. urn:nbn:hr:178:559977
Bach, Ana
University of Zagreb
Faculty of Veterinary Medicine
Department of Animal Nutrition and Dietetics

Institutional repository: Repository of Faculty of Veterinary Medicine

Cite this document

Bach, A. (2015). Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:559977

Bach, Ana. "Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:178:559977

Bach, Ana. "Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:178:559977

Bach, A. (2015). 'Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:559977

Bach A. Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2015 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:178:559977

A. Bach, "Učinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:178:559977

Metadata
TitleUčinkovitost manan oligosaharida (Bio MosR) kao zamjene antibiotskim promotorima rasta u odbijene prasadi
Title (english)Effect of Mannan-Oligosaccharide (Bio MosR) as an Alternative to Antibiotic Growth Promoters in Weaned Pigs
AuthorAna Bach
MentorHrvoje Valpotić (mentor)
Committee memberTomislav Mašek
Committee memberMarko Samardžija
Committee memberHrvoje Valpotić
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Animal Nutrition and Dietetics)
Zagreb
Defense date and country2015-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Basic and Preclinical Veterinary Sciences
Abstract
Cilj ovog istraživanja je istražiti učinkovitost mannan oligosaharida dobivenog is stanične stijenke kvasca kao moguće zamjene antibiotskim promotorima rasta u hranidbi odbijene prasadi. Pokus je proveden na 46 prasadi (28 dana stare sa 7.25±0.15 kg TM) koja je podjeljena na kontrolnu i pokusnu skupinu i hranjena komercijalnom smjesom bez antibiotika ili ostalih antimikrobnih preparata tijekom 5 tjedana nakon odbića. Pokusna skupina je u smjesi dobivala 0.2% pripravka (BioMos®) tijekom cijelog perioda istraživanja. Uzorke krvi za protočnu citometriju uzimali smo tjedno od 7 prasadi od koje smo 0., 14., 21. i 35. dana pokusa uzimali i rektalne obriske za bakteriološku pretragu. Prasad je svakodnevno promatrana radi uočavanja pojave i jačine proljeva ili dugih kliničkih znakova poremećaja zdravstvenog stanja. Sva prasad i hrana je vagana tjedano radi praćenja proizvodnih pokazatelja. Skupina prasadi tretirana MOS-om imala je nižu prosječnu TM (16,65 kg što je za 5,45 % niže od kontrolne prasadi) na kraju istraživanja, uz nešto nižu konverziju (2,01) od kontrole (2,17) te nešto slabiji PDP/PDUH (268g/539g) u odnosu na kontrolu (296g/641g) tijekom 5 tjedana trajanja pokusa. Prasad iz kontrolne skupine imala je najveću pojavnost proljeva (12/23 ili 52%), dok je MOS-om tretirana prasad imala znatno nižu pojavnost proljeva (6/23 ili 26%). PJP je bila najviša u kontrolne prasadi (1,54), dok je prasad u skupini tretiranoj MOS-om imala nešto manju PJP (1,23). Međutim, broj uginuća bio je veći u skupini prasadi tretiranoj MOS-om (5/23 ili 21%) u usporedbi s kontrolom (4/23 ili 17%). Statistički značajno više (P<0.01) vrijednosti udjela CD45+ stanica u odnosu na kontrolu zabilježili smo od 21. do 35. dana; CD4+ pomoćničkih T limfocita (Th), između 28. i 35. dana (P<0.01); CD8+ citolitičkih T limfocita (Tc) između 21. i 28. Dana (P<0.01); CD4+CD8+ T limfocita 28. dana(P<0.05); CD21+ B stanica između 21. i 35 dana (P<0.01). Testirani pripravak je bio najučinkovitiji u pojačavanju nespecifične sustavne imunosti te smanjenju pojavnosti I jačine proljeva, međutim nije pokazao učinkovitost kao promotor rasta, s izuzetkom postizanja niže konverzije hrane.
Abstract (english)
Aim of this investigation was to evaluate the efficiency of mannan oligosaccharide obtained from the yeast cell wall as a possible alternative to antibiotic growth promoters in diets of weaned pigs. Trial was conducted on 46 piglets (28 days old with 7.25±0.15 kg BW) that were assigned to control and experimental group and fed a commercial feed mixture without antibiotics and other antimicrobials during 5 weeks after weaning. Experimental group received feed with 0.2% of BioMos® during the entire trial. Blood samples for flow cytometry were collected from 7 pigs from which rectal swabs for bacteriology were also taken on days 0., 14., 21., and 35. Pigs were monitored daily for onset of diarrhea and other clinical signs of illness. All pigs and feed were weighed weekly for the monitoring of performance. Piglets treated with MOS had lower average BW (16,65 kg which is 5,54% lower from control); better feed conversion (2,01 compared to 2,17); and lower ADG/ADFI (268/539 g compared to 296/641 g) during 5 weeks of the experiment. Control piglets had the most diarrhea incidence (12/23 or 52%), while the treated piglets had (6/23 or 26%). Average diarrhea score was higher in the non treated piglets (1,54) compared to (1,23) of the treated piglets. However, overall mortality was higher in the treated group (5/23 or 21%) compared to non-treated piglets (4/23 or 17%). Significantly higher (P<0.01) values of CD45+ cells were recorded in treated pigs from days 21. to 35.; CD4+ T helper lymphocytes (Th), from days 28. to 35. (P<0.01); CD8+ cytolytic T lymphocytes (Tc) from days 21. to 28. (P<0.01); CD4+CD8+ T lymphocytes on day 28. (P<0.05); and CD21+ B cells from days 21. To 35. (P<0.01). Mannan oligosaccharide was most efficient in increasing the level of non specific system immunity and the reduction of incidence and severity of diarrhea. However it did not increase performance of treated animals with the exception of decreasing feed conversion ratio.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:559977
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-01-27 11:17:56