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master's thesis
Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2016. urn:nbn:hr:178:326774
Rajković, Tomislav
University of Zagreb
Faculty of Veterinary Medicine
Department of Hygiene and Technology of Foodstuffs

Institutional repository: Repository of Faculty of Veterinary Medicine

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Rajković, T. (2016). Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:326774

Rajković, Tomislav. "Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:178:326774

Rajković, Tomislav. "Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:178:326774

Rajković, T. (2016). 'Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:326774

Rajković T. Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2016 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:178:326774

T. Rajković, "Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:178:326774

Metadata
TitleOdređivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa
Title (english)Determination of Antimicrobial Susceptibility of Listeria Monocytogenes from Food by E-Test
AuthorTomislav Rajković
MentorNevijo Zdolec (mentor)
Committee memberVesna Dobranić (predsjednik povjerenstva)
Committee memberIvana Filipović (član povjerenstva)
Committee memberNevijo Zdolec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Hygiene and Technology of Foodstuffs)
Zagreb
Defense date and country2016-06-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Abstract
U radu je istražena osjetljivost na antimikrobne tvari bakterije Listeria monocytogenes (n=26) izoliranih iz hrane i proizvodnih pogona primjenom E-testa. Određene su MIC vrijednosti ampicilina, gentamicina, vankomicina, streptomicina, linezolida, azitromicina, rimfapicina, oksacilina, trimetoprim/sulfametoksazola i ciprofloksacina. Svi su izolati bili osjetljivi na klinički relevantne antimikrobne lijekove gentamicin, streptomicin, azitromicin, rifampicin, trimetoprim/sulfametoksazol i ciprofloksacin. Distribucija izolata L. monocytogenes prema MIC vrijednostima bila je za većinu tvari u nižem rasponu u odnosu na EUCAST podatke. Pet izolata (19,23 %) L. monocytogenes bilo je otporno na najmanje jednu tvar, a svi su izolirani s radnih površina, sirovine i opreme u mesnoj industriji. Dva su izolata bila otporna na vankomicin, po tri na linezolid i oksacilin, a jedan na sve tri tvari. Jedan izolat je pokazao otpornost na vankomicin, linezolid i ampicilin. Također, u otpornih izolata izmjerene su i visoke MIC vrijednosti za azitromicin-rifampicin, ampicilin-oksacilin, odnosno streptomicin-linezolid. Rezultati ukazuju na nisku razinu otpornosti L. monocytogenes iz hrane na klinički relevantne antimikrobne lijekove. S druge strane nalaz višestruko otpornih L. monocytogenes na radnim površinama i opremi u mesnoj industriji upućuje na rizik prijenosa antimikrobne rezistencije kroz lanac proizvodnje hrane.
Abstract (english)
In this study the antimicrobial susceptibility of Listeria monocytogenes from food and food processing facilities (n=26) was tested by E-test. Minimal inhibitory concentrations of ampicillin, gentamicin, vancomycin, streptomycin, linezolid, azitromycin, rifampicin, oxacillin, trimethoprim/sulphametoxazol and ciprofloxacin were measured. All isolates were susceptible to clinically relevant antibiotics gentamicin, streptomicin, azitromycin, rifampicin, trimethoprim/sulphametoxazol and ciprofloxacin. Distribution of L. monocytogenes according to MIC levels ranged for most agents in lower values compared to EUCAST data. Five isolates (19.23 %) of L. monocytogenes was resistant at least to one antimicrobial agents, and all originated from raw material, food contact surfaces and equipment in meat processing plant. Two isolates were resistant to vancomycin, three to linezolid and oxacillin, and one to all three agents. One isolate showed the resistance to vancomycin, linezolid and ampicillin. Also, in the case of resistant isolates, the high-level MIC of azitromycin-rifampicin, ampicillin-oxacillin and streptomicinlinezolid were recorded. Results show the low level of resistance toward clinically relevant antimicrobials in L. monocytogenes from food. Contrary, the occurrence of multi-resistant L. monocytogenes strains in meat-processing facility samples indicates the risk of antimicrobial resistance transfer along the food producing chain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:326774
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-23 08:57:21