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master's thesis
Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2018. urn:nbn:hr:178:916778
Zemljić, Sara
University of Zagreb
Faculty of Veterinary Medicine
Department of Poultry Diseases with Clinic

Institutional repository: Repository of Faculty of Veterinary Medicine

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Zemljić, S. (2018). Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:916778

Zemljić, Sara. "Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:178:916778

Zemljić, Sara. "Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:178:916778

Zemljić, S. (2018). 'Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:916778

Zemljić S. Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2018 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:178:916778

S. Zemljić, "Trajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:178:916778

Metadata
TitleTrajanje stanične imunosti u kokoši lake pasmine za virus newcastleske bolesti određene stimulacijom leukocita in vitro
Title (english)Duration of Cell-Mediated Immunity for Newcastle Disease in Laying Hens Determined by in vitro stimulation of Leukocytes
AuthorSara Zemljić
MentorŽeljko Gottstein (mentor)
MentorGordana Nedeljković (mentor)
Committee memberSelma Pintarić (predsjednik povjerenstva)
Committee memberGordana Nedeljković (član povjerenstva)
Committee memberŽeljko Gottstein (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Poultry Diseases with Clinic)
Zagreb
Defense date and country2018-02-05, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Abstract
Newcastleska bolest (NB) je izuzetno kontagiozna virusna zarazna bolest kokoši i drugih vrsta ptica. Kontrola bolesti se provodi cijepljenjem i nespecifičnim mjerama. Kako bi se izmjerila učinkovitost postojećeg cjepnog programa na proizvodnoj farmi može se paralelno koristiti procjena humoralne i stanične imunosti. Cilj ovog rada je opisati metode kojima se može odrediti stanična imunost u određenim vremenskim razmacima od cijepljenja i time njeno trajanje. Stanična imunost određuje se mjerenjem in vitro proliferacije imunosnih stanica nakon stimulacije uz određivanje razine citokinskog odgovora.Od metoda mogu se koristiti protočna citometrija, imunoenzimni test, ELISPOT test, a od molekularnih metoda lančana reakcija polimerazom u stvarnom vremenu. U rutinskoj dijagnostici kao najprikladnija metoda pokazao se PCR zbogjednostavnosti i brzine izvedbe. Stanični imunosni odgovor je veoma bitan za ranu i cijepljenjem potaknutu zaštitu organizma, međutim sam nije dostatan da zaštiti ptice od VNB.Dostupnaistraživanja ne ukazuju na stvarno stanje in vivo te je potrebno provesti dodatna istraživanja.
Abstract (english)
Newcastle disease (ND) is very contagious viral disease of poultry and other birds. Control of ND is achieved by vaccination and non-specific measures. The efficiency of vaccination programs implemented on farms is assessed by testing humoral and cell-mediated immunity (CMI). The aim of this paper was to describe the methods used for determination of CMI in certain time points after vaccination (dpv), and thus, duration of CMI. Cell-mediated immunity can be determined by in vitro stimulation and proliferation of peripheral blood mononuclear cells (PBMCs), and the level of cytokine response/release. Flow cytometry, ELISA, ELISPOT, and Real -Time PCR are mostly used in CMI assessment. Real-Time PCR has been proven to be the most appropriate method for routine diagnostic, due to simplicity and speed of the procedure. CMI plays a crucial role in early immune response following immunisation. However, it's not sufficient to protect by itself the birds from NDV infection. Available studies so far don't reveal the true in vivo status, therefore further research is required.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:916778
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-23 09:40:58