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master's thesis
Diferencijacija stanica u primarnoj kulturi neurona
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2016. urn:nbn:hr:178:061182
Mirić, Mladen
University of Zagreb
Faculty of Veterinary Medicine
Department of Anatomy, Histology and Embriology

Institutional repository: Repository of Faculty of Veterinary Medicine

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Mirić, M. (2016). Diferencijacija stanica u primarnoj kulturi neurona (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:061182

Mirić, Mladen. "Diferencijacija stanica u primarnoj kulturi neurona." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:178:061182

Mirić, Mladen. "Diferencijacija stanica u primarnoj kulturi neurona." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:178:061182

Mirić, M. (2016). 'Diferencijacija stanica u primarnoj kulturi neurona', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:178:061182

Mirić M. Diferencijacija stanica u primarnoj kulturi neurona [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:178:061182

M. Mirić, "Diferencijacija stanica u primarnoj kulturi neurona", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:178:061182

Metadata
TitleDiferencijacija stanica u primarnoj kulturi neurona
Title (english)Cell Differentiation in Primary Neuronal Culture
AuthorMladen Mirić
MentorSnježana Kužir (mentor)
MentorIvan Alić (mentor)
Committee memberTajana Trbojević Vukičević (predsjednik povjerenstva)
Committee memberMartina Đuras (član povjerenstva)
Committee memberSnježana Kužir (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Anatomy, Histology and Embriology)
Zagreb
Defense date and country2016-12-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Abstract
Glavni cilj ovoga istraživanja bio je napraviti primarnu kulturu neurona podrijetlom iz dva mišja soja kroz 7 i 14 dana, opisati uzorak izražaja bjelančevine te ih međusobno usporediti. Stanice mišjeg soja GFP-LUC tijekom sedmog dana su se diferencirale u potpunosti u zrele neurone i astrocite, od kojih je GFP pozitivan u 100% diferenciranih stanica, dok su biljezi neurona (MAP2, ß3-tubulin i NeuN) pozitivni u 90% diferenciranih stanica, a biljeg astrocita GFAP u samo 10%, odnosno tijekom četrnaestog dana su stanice razvijenije (veći broj i deblji nastavci), a biljezi neurona su pozitivni u 80% diferenciranih stanica, a biljeg astrocita u 20%. Stanice mišjeg soja Thy1-YFP tijekom sedmog dana su se također u potpunosti diferencirale u zrele neurone i astrocite, međutim ovdje je samo 20% neurona YFP pozitivno. Kao i kod GFPLUC mišjeg soja, i ovdje je u kulturi prisutno 90% neurona i 10% astrocita, odnosno 80% neurona i 20% astrocita tijekom četrnaestog dana.
Abstract (english)
The main goal of this study was to differentiate a primary culture of neurons derived from two different mouse strains through 7 and 14 days, to describe the pattern of protein expression, and compare them with each other. Cells derived from GFP-LUC mouse strain during the 7th day were completely differentiated into mature neurons and astrocytes and they were 100% GFP positive; 90% were neurons and express neuronal markers (MAP2, ß3-tubulin and NeuN) while the 10% were astrocytes and express GFAP. On the 14th day of differentiation 80% express neuronal markers while 20% express GFAP. On the other hand, cell derived from Thy1-YFP mouse strain were also completely differentiated on the 7th day, but only 20% neurons express Thy1-YFP. Similar, like GFP-LUC cells on the 7th day we observed 90% neurons and 10% astrocytes in cell culture, while on the 14th day percentage of neurons decrease at 80% while percentage of astrocytes increase on 20%.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:061182
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-24 11:10:03