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master's thesis
Ekspresija E i N kadherina u tumorima sjemenika pasa
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2018. urn:nbn:hr:178:855839
Mlinarić, Mirna
University of Zagreb
Faculty of Veterinary Medicine
Department of Veterinary Pathology

Institutional repository: Repository of Faculty of Veterinary Medicine

Cite this document

Mlinarić, M. (2018). Ekspresija E i N kadherina u tumorima sjemenika pasa (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:855839

Mlinarić, Mirna. "Ekspresija E i N kadherina u tumorima sjemenika pasa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:178:855839

Mlinarić, Mirna. "Ekspresija E i N kadherina u tumorima sjemenika pasa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:178:855839

Mlinarić, M. (2018). 'Ekspresija E i N kadherina u tumorima sjemenika pasa', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:855839

Mlinarić M. Ekspresija E i N kadherina u tumorima sjemenika pasa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2018 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:178:855839

M. Mlinarić, "Ekspresija E i N kadherina u tumorima sjemenika pasa", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:178:855839

Metadata
TitleEkspresija E i N kadherina u tumorima sjemenika pasa
Title (english)Expression of E and N Cadherins in Canine Testicle Tumors
AuthorMirna Mlinarić
MentorMarko Hohšteter (mentor)
MentorAndrea Gudan Kurilj (mentor)
Committee memberBranka Artuković (predsjednik povjerenstva)
Committee memberAndrea Gudan Kurilj (član povjerenstva)
Committee memberMarko Hohšteter (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Veterinary Pathology)
Zagreb
Defense date and country2018-04-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Abstract
Tumori sjemenika su najčešće neoplazme reproduktivnog sustava pasa i najčešće se javljaju kod starijih životinja. Kadherini su transmembranski glikoproteini, ovisni o kalciju, koji spadaju u skupinu staničnih adhezivnih molekula. Od osamdesetak poznatih kadherina, najznačajniji su epitelni (E-kadherin) i neuralni (N-kadherin). E-kadherin funkcionira kao supresor invazije tumora te je njegova ekspresija smanjena u većini tumora, dok je N-kadherin često reguliran kao promotor invazije tumora. U ovom radu provedena je usporedna imunohistokemijska analiza tumorskih biljega E- i N-kadherina te histopatološka analiza arhiviranih uzoraka tumora sjemenika pasa. Cilj je bio odrediti prisutnost i distribuciju transmembranskih glikoproteina E- i N-kadherina u odabranim uzorcima tumora sjemenika pasa. Od ukupno 13 uzoraka, histopatološkom analizom utvrđeno je 10 jednostavnih tumora i to seminoma (7 difuznih i 3 intratubularna seminoma) te 3 mješovita tumora (mješoviti tumor Leydigovih stanica i difuzni seminom, mješoviti tumor Leydigovih i Sertolijevih stanica, te mješoviti tumor Leydigovih stanica i intratubularni seminom). Ekspresija E- i N-kadherina očitovala se u svim analiziranim tumorozno promijenjenim uzorcima sjemenika pasa te je u svih analiziranih tumora ustanovljen izraženiji intenzitet obojenja u većem postotku ekspirimiranih stanica N-kadherina u odnosu na E-kadherin. Kod seminoma je utvrđena slabija ekspresija E-kadherina i jača ekspresija N-kadherina u odnosu na tumor Leydigovih i tumor Sertolijevih stanica. Također, utvrđena je jača ekspresija E-kadherina kod intratubularnih u odnosu na difuzne seminome. Incidencija tumora sjemenika pasa je sve veća te postoji potreba za dodatnim imunohistokemijskim analizama i pronalaženju morfoloških i bioloških obilježja tumora koja bi se mogla koristiti u svrhu prognoziranja i optimizaciji terapije tumora sjemenika pasa. Analiza imunohistokemijskih biljega E- i N-kadherina može biti od pomoći u dijagnostici tumora sjemenika pasa, osobito ako se koristi uz histopatološku pretragu.
Abstract (english)
Testicular tumors are the most common neoplasms of the reproductive system in dogs and they most commonly occur in older animals. Kadherins are calcium dependent transmembrane glycoproteins belonging to a group of cell adhesion molecules. Of the eighty known cadherins, the most significant are epithelial ( E-cadherin) and neural (N-cadherin). E-cadherin functions as a suppressor of tumor invasion and it's expression is reduced in most tumors, while N-cadherin is often regulated as a tumor invasion promoter. In this research a histopathological and parallel immunohistochemical analysis of tumor markers of E- and N-cadherin on canine testicular tumors was performed. Aim of this study was to determine the presence and distribution of tumor markers E- and N-cadherin in selected samples of dog testicular tumors. The histopathological analysis of 13 testicular tumors samples showed 10 simple tumors, namely seminoma (7 diffuse and 3 intratubular seminomas), and 3 mixed tumors (mixed tumor of Leydig cell and diffuse seminoma, mixed tumor of Leydig and Sertolian cells and mixed tumor of Leydig cell and intratubular seminoma). Expression of E- and N-cadherin was revealed in all analyzed tumor-modified samples of canine testicular tumors, also in all analyzed tumors was found a higher intensity of coloration and a higher percentage of expressed N-cadherin in cells compared to E-cadherin. Lower E-cadherin and stronger N-cadherin expression was found in seminoma compared to Leydig cell tumors and Sertoli tumor cells. Also, stronger expression of E-cadherin was found in intratubular compared to diffuse seminoma. The incidence of dog testicular tumors is increasing and there is a need for further immunohistochemical evaluations and search for biological feautures of the tumor that would be of benefit in optimizing canine testicular tumor therapy. The analysis of immunohistochemical markers of E- and N-cadherin combined with histopathological examination has the capacity to improve diagnosis of canine testicular tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:855839
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-25 05:58:27