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master's thesis
Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2017. urn:nbn:hr:178:170016
Šokičić, Mira
University of Zagreb
Faculty of Veterinary Medicine
Department of Pathophysiology

Institutional repository: Repository of Faculty of Veterinary Medicine

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Šokičić, M. (2017). Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:170016

Šokičić, Mira. "Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:178:170016

Šokičić, Mira. "Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:178:170016

Šokičić, M. (2017). 'Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:178:170016

Šokičić M. Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:178:170016

M. Šokičić, "Utjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:178:170016

Metadata
TitleUtjecaj in vitro stimulacije perifernih mononuklearnih stanica krvi konkavalinom A na morfometrijske karakteristike limfocita
Title (english)The Influence of In Vitro Stimulation of Peripheral Mononuclear Blood Cells with Concavaline A on Morphometric Characteristics of Lymphocytes
AuthorMira Šokičić
MentorNina Poljičak-Milas (mentor)
MentorKristina Matković (mentor)
Committee memberMirna Robić (predsjednik povjerenstva)
Committee memberKristina Matković (član povjerenstva)
Committee memberNina Poljičak-Milas (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Pathophysiology)
Zagreb
Defense date and country2017-06-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Abstract
Opća proliferativna sposobnost T-limfocita procjenjuje se invitro stimulacijom biljnim mitogenima, fitohemaglutininom (PHA) i konkavalinom A (ConA). Promjene nastale izlaganjem mitogenima i stimulacijom imunosnog sustava, mogu dovesti do povećanja i/ili abnormalnosti stanice i jezgre, te promjene njihovog meĊusobnog odnosa, što se moţe kvantificirati morfometrijom. Na ukupno 18 krvnih razmaza mjerene su morfometrijske karakteristike limfocita prije i nakon aktivacije konkavalinom A. Digitalna analiza krvnih stanica napravljena je na osobnom raĉunalu s podrţavajućim programom SFORM (VAMSTEC, Zagreb). Rezultati su pokazali znaĉajno povećanje površine, opsega, maksimalnog polumjera, konveksne površine te faktora pravilnosti limfocita nakon inkubacije s mitogenom u usporedbi s istim pokazateljima stanice limfocita prije inkubacije. Vrijednosti minimalnog polumjera i duţine stanice bile su podjednake u limfocitima prije i nakon inkubacije. Kod mjerenja morfometrijskih pokazatelja jezgara, nisu izmjerene znaĉajne promjene njihove površine ili opsega, meĊutim utvrĊen je znaĉajno smanjem minimalni polumjer i širina jezgre, znaĉajno manji N/C omjer te smanjena vrijednost faktora oblika (FF). Dobiveni rezultati pokazali su da promjene na stanicama limfocita i njihovim jezgrama upućuju na metaboliĉku aktivnost pri tretiranju mitogenima te morfometrijska analiza moţe pruţiti vrijedne podatke u procjeni transformacije imunokompetentnih stanica.
Abstract (english)
General proliferative ability of T-cells estimates with in vitro stimulation with plant mitogens, phytohemaglutinin (PHA) and concavalin A (ConA). Changes caused by exposure to mitogens and stimulation of the immune system may lead to an increase and/or abnormality of the cell and nucleus, and a change in their mutual relationship, which can be quantified by morphometry. At the total of 18 blood smears the morphometric characteristics of the lymphocytes before and after activation with concavalin A were measured. The digital blood cell analysis was performed on a personal computer using the support program SFORM (VAMSTEC, Zagreb). The results showed a significant increase in surface, bandwidth, maximum radius, convex surface, and lymphocyte form factor after incubation with mitogen compared to the same lymphocyte cell pre-incubation ratios. The minimum radius and cell length values were consistent in lymphocytes before and after incubation. When measuring the morphometric parameters of the nuclei, no significant changes in their surface or extent were measured, however, there was significant reduction in the minimum radius and core width, significantly lower N / C ratio and reduced factor factor (FF). The obtained results showed that changes in lymphocyte cells and their nuclei indicate metabolic activity in the treatment of mitogens, and morphometric analysis can provide valuable data in estimating the transformation of immunocompetent cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:170016
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-25 06:30:57