| Abstract | Bakterije roda Campylobacter sp. imaju važnu ulogu u etiologiji zaraznih enteritisa
pasa, drugih vrsta životinja, ali i ljudi. Životinje koje izlučuju ovu bakteriju izmetom
predstavljaju izvor infekcije za druge životinje i ljude.
Kampilobakterioza je zoonoza, bolest koja se na čovjeka prenosi sa životinja i
proizvoda životinjskog podrijetla. Iako se meso peradi i goveda smatra glavnim izvorom
bolesti, izvor također može biti i izmet pasa, a bakteriju mogu izlučivati i zdravi psi. Izvor
infekcije za pse predstavljaju kontaminirana hrana, voda i okoliš, kontakt s izmetom
inficiranih životinja, a zaraza se širi feko oralno. Čimbenici rizika od infekcije u pasa su
izloženost vodama stajačicama te život u aglomeracijama poput azila i uzgajivačnica koji
omogućuje lakše širenje infekcije. Veća učestalost kliničkog očitovanja bolesti u štenadi i
mladih pasa je najvjerojatnije posljedica slabijeg razvitka imunog sustava. Klinički bolest se
očituje nespecifičnim znakovima gastroenteritisa, a infekcija može biti i subklinička, kada
životinje izlučuju uzročnika izmetom. Kampilobakterioza je vodeća bakterijska zoonoza
povezana s trovanjem hranom u zemljama Europske unije, dok je u Hrvatskoj i dalje na
prvom mjestu salmoneloza, ali je kampilobakterioza odmah na drugom mjestu.
Ciljevi ovog istraživanja bili su utvrditi i usporediti učestalosti izdvajanja
kampilobaktera iz zdravih pasa i pasa s proljevom, utvrditi i usporediti učestalosti izdvajanja
kampilobaktera unutar dobnih skupina, te identificirati vrstu izdvojenih kampilobaktera.
U istraživanje određivanja učestalosti izlučivanja bakterija roda Campylobacter u pasa
uključeno je 70 pasa s proljevom i 70 zdravih pasa te su podijeljeni u dvije dobne skupine:
mlađe od godinu dana i starije od godinu dana. 24
Od ukupno 140 pretraživanih uzoraka izmeta pasa, iz njih 36,4% su izdvojene bakterije roda
Campylobacter. U skupini pasa s proljevom 67,1% pasa je bilo pozitivno, a u skupini zdravih
pasa 5,7%. Značajno je češće izdvojen iz pasa sa proljevom nego iz zdravihpasa (p<0,01). U
skupini zdravih pasa češće je izdvojen iz pasa mlađih od godine dana (p<0,01). U skupini
pasa s proljevom nije bilo razlike između dobnih skupina. Svi pohranjeni izolati porijeklom
od pasa s proljevom identificirani su MALDI-TOF metodom kao vrsta C. jejuni. Rezultati
istraživanja govore u prilog tome da je učestalost izlučivanja kampilobaktera u pasa velika,
posebice u pasa s proljevom. Unutar skupine zdravih životinja veća učestalost izlučivanja
uzročnika utvrđena je u pasa mlađih od godinu dana, što treba imati na umu posebice ako
životinje dolaze u kontakt s djecom ili imunokompromitiranim osobama. |
| Abstract (english) | Campylobacter sp. have an important role in the etiology of contagious enteritis of
dogs, other species of animals, but also of humans. Animals that excrete this bacterium by
feces are a source of infection for other animals and humans.
Campylobacteriosis is zoonoses, a disease that is transmitted to humans from animals
and animal products. Although poultry and cattle are considered the main source of disease,
the source can be feces of dogs and also bacterium can be excreted by healthy dogs. The
source of the infection in dogs is contaminated food, water, environment, contact with feces
of infected animals and the infection spreads faeco oral way. Factors of the risk of infection in
dogs are exposure to water retention and life in agglomerations such as asylum and kennel
that makes it easier to spread the infection. The higher incidence of clinical manifestation of
disease in puppies and young dogs is most likely a consequence of weaker immune system
development. Clinical illness is manifested by nonspecific signs of gastroenteritis, and the
infection may be subclinical when animals excrete Campylobacter sp. by faeces.
Campylobacteriosis is the leading bacterial zoonosis associated with food poisoning in
European Union countries, while in Croatia in the first place is salmonella, but
campylobacteriosis is in the second place.
The aim of this study was to establish and compare the frequency of separation of
campylobacter from healthy dogs and dogs with diarrhea, to determine and compare the
frequency of campylobacter isolation within age groups and to identify the type of isolated
campylobacter. 26
In the study of the rate of excretion of bacterial strains of Campylobacter in dogs, 70
dogs with diarrhea and 70 healthy dogs were included and were divided into two age groups:
younger than one year and older than one year.
Out of a total of 140 examined samples of feces, 36.4% tested positive. In the group of dogs
with a diarrhea, 67.1% was positive and in the group of healthy dogs 5.7%. It is significantly
more common in dogs with diarrhea than in healthy dogs (p <0.01). In the group of healthy
dogs it is more commonly isolated from dogs under the age of one year (p <0.01). In dogs
with diarrhea there was no difference between the age groups. All stored isolates of dogs with
diarrhea were identified by the MALDI-TOF method as C. jejuni species. The results of the
study suggest that the incidence of Campylobacter sp. shedding in dogs is high, especially in
diarrheaic dogs. Within the group of healthy animals, a higher incidence of shedding has been
found in dogs under one year old. That should be kept in mind, especially when animals come
into contact with children or immunocompromised people |
