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master's thesis
Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2019. urn:nbn:hr:178:545203
Papak, Ines
University of Zagreb
Faculty of Veterinary Medicine
Department of Microbiology and Infectious Diseases with Clinic

Institutional repository: Repository of Faculty of Veterinary Medicine

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Papak, I. (2019). Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:545203

Papak, Ines. "Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:545203

Papak, Ines. "Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:545203

Papak, I. (2019). 'Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:545203

Papak I. Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2019 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:178:545203

I. Papak, "Utvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:178:545203

Metadata
TitleUtvrđivanje prisutnosti bakterije Anaplasma Phagocytophilum u populaciji mišolikiih glodavaca
Title (english)Prevalence of Anaplasma Phagocytophilum in Wild Rodent Population
AuthorInes Papak
MentorSuzana Hađina (mentor)
Committee memberZrinka Štritof (predsjednik povjerenstva)
Committee memberJosipa Habuš (član povjerenstva)
Committee memberSuzana Hađina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Microbiology and Infectious Diseases with Clinic)
Zagreb
Defense date and country2019-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Clinical Sciences
Abstract
Anaplazmoza je zarazna bolest koju uzrokuju bakterije iz roda Anaplasma. A. phagocytophilum uzročnik je anaplazmoze konja, krpeljne groznice preživača, granulocitne anaplazmoze pasa i mačaka te humane granulocitne anaplazmoze u ljudi. Srna i divlji glodavci prirodni su rezervoari A. phagocytophilum, a osim toga glodavci su i domaćini svih razvojnih stadija krpelja. Cilj ovog istraživanja bio je utvrditi prisutnost bakterije A. phagocytophilum u populaciji mišolikih glodavaca. Uzorci tkiva slezene pretraženi su ugniježđenom lančanom reakcijom polimeraze, te su pozitivni rezultati utvrđeni u 15% uzoraka. U svrhu provjere utvrđivanja vrste A. phagocytophilum parovima početnica EC9/EC12a i SSAP2f/SSAP2r korištenih u ovom istraživanju, nasumično je odabrano pet pozitivnih uzoraka te poslano na sekvenciranje. Sekvence su analizirane BLAST programom kojim je utvrđena 100% podudarnost s bakterijskom vrstom iz porodice Anaplasmataceae, Candidatus Neoehrlichia mikurensis. Rezultati ovog preliminarnog istraživanja pokazali su da se bez sekvenciranja svih pozitivnih uzoraka te korištenja više različitih parova početnica ne može utvrditi stvarna prisutnost bakterije A. phagocytophilum. Kod određenih životinjskih vrsta na određenom području javljaju se unakrižne reakcije s drugim bakterijskim vrstama iz porodice Anaplasmataceae.
Abstract (english)
Anaplasmosis is an infectious disease caused by bacteria from the genus Anaplasma. A. phagocytophilum is the cause of equine anaplasmosis, ruminant tick fever, canine and feline granulocytic anaplasmosis, and human granulocytic anaplasmosis. Deer and wild rodents are natural reservoirs of A. phagocytophilum, and in addition, rodents are also host tick development stages. The aim of this study was to determine the presence of A. phagocytophilum in a wild rodent population. Spleen tissue samples were examined by nested polymerase chain reaction and positive results were found in 15% of the samples. For the purpose of confirming the determination of A. phagocytophilum by EC9/EC12a and SSAP2f/SSAP2r primers used in this study, five positive samples were randomly selected and sent for sequencing. The sequences were analyzed by a BLAST program that determined 100% identity with Candidatus Neoehrlichia mikurensis, a bacterial species belonging to the family Anaplasmataceae,. The results of this preliminary study showed that without sequencing all positive samples and using different primer sets the presence of A. phagocytophilum could not be determined. In certain animal species in certain geographic area there is a possibility of cross-reactions occurring with other bacterial species in the family Anaplasmataceae.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:545203
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-01-28 12:49:06