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master's thesis
Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2019. urn:nbn:hr:178:011299
Vukušić, Nina
University of Zagreb
Faculty of Veterinary Medicine
Department of Hygiene and Technology of Foodstuffs

Institutional repository: Repository of Faculty of Veterinary Medicine

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Vukušić, N. (2019). Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:011299

Vukušić, Nina. "Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:011299

Vukušić, Nina. "Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:011299

Vukušić, N. (2019). 'Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 14 November 2024, https://urn.nsk.hr/urn:nbn:hr:178:011299

Vukušić N. Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2019 [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:178:011299

N. Vukušić, "Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:178:011299

Metadata
TitleKarakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla
Title (english)Characterisation of Bacteriocins Produced by Enterococci Isolated from Food of Animal Origin
AuthorNina Vukušić
MentorNevijo Zdolec (mentor)
Committee memberVesna Dobranić (predsjednik povjerenstva)
Committee memberŽeljka Cvrtila (član povjerenstva)
Committee memberNevijo Zdolec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Hygiene and Technology of Foodstuffs)
Zagreb
Defense date and country2019-01-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Animal Production and Biotechnology
Abstract
Enterokoki imaju važnu ulogu u fermentaciji hrane te često pokazuju probiotička svojstva. U ovom radu istražen je njihov antimikrobni učinak in vitro prema referentnim sojevima i sojevima izoliranih iz hrane bakterija Listeria monocytogenes, Listeria ivanovii, Listeria innocua, Yersinia enterocolitica i Salmonella Typhimurium. Korištena je agar difuzijska metoda, a nadtalozi neutralizirani i tretirani proteazom. Enterococcus faecalis EF-101 je pokazao snažno inhibicijsko djelovanje prema svim sojevima bakterije roda Listeria, kao i soja Y. enterocolitica O:3. Karakterizacijom inhibitora utvrđeno je da se radi o tvari proteinske strukture, moguće bakteriocina enterocina čija je aktivnost bila 1280 AU/ml. Potrena su daljnja istraživanja radi potencijalne primjene enterocina kao prirodnog konzervansa u očuvanju održivosti hrane i povećanja mikrobiološke sigurnosti.
Abstract (english)
Enterococci have an important role in food fermentation and often exhibit probiotic properties. In this paper, their antimicrobial effect was investigated in vitro against reference strains and strains isolated from the food - Listeria monocytogenes, Listeria ivanovii, Listeria innocua, Yersinia enterocolitica and Salmonella Typhimurium. The agar diffusion method was used, and the supernatants were neutralized and treated with protease. Enterococcus faecalis EF-101 showed strong inhibitory activity against all strains of Listeria as well as Y. enterocolitica O:3 strain. By the characterization of the inhibitor, proteinaceous nature was found, a possible bacteriocin enterocin, whose activity was 1280 AU / ml. Further research should be carried out to assess the potential use of enterocin as a natural preservative in food conservation and protection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:011299
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-11-02 07:31:00