Mastitis is an inflammation of the mammary gland caused by the entry of microorganisms through the suction canal and mastering the defense mechanisms of the udder. It has a significant impact on the production and reproduction of dairy cattle and is considered to be the most important and costly disease in the dairy industry. In order to be able to calculate in detail the total cost of mastitis on a farm, we had to take into account all the costs borne by the disease itself, plus the cost of preventive measures and the cost of disease control. Mastitis is caused by a wide variety of microorganisms and it occurs in the form of a subclinical or clinical infection. The decline in milk yield of cows due to clinical or subclinical mastitis is considered to be the most important economic factor of the disease. Reduced milk production in one cow may be associated with an increased number of somatic cells in milk due to subclinical mastitis. The higher number of somatic cells, the lower the milk production and vice versa. In addition to the decline in milk production, it must also be pointed the high costs of discarded milk that are thrown away during and after antibiotic use, until the withdrawal period expires. The cost of this discarded milk is a huge expense, so manufacturers are trying to minimize the use of antibiotics and withdrawal drugs. In addition, the overuse of antimicrobials has greatly contributed to the resistance of microorganisms. The scientists are working hard on the solutions that could reduce at least some of the antibiotics that are used. Hedera d.o.o. (Split, Croatia) as part of a project and collaboration with the Faculty of Veterinary Medicine in Zagreb has developed an innovative, non-alcoholic intra mammary formulation based on Propolis and phenolic acids called Apimast. The main advantages of this formulation are the lack of withdrawal time and most likely the inability of microorganisms to develop microbial resistance. In this paper, using the bioeconomic modeling, a simulation of income and costs was performed on an average EU farm with and without using Apimast. A software tool called the "Cost of Mastitis Calculation Tool for EU28" was used to calculate all costs of mastitis on a farm in the EU. 42 In this paper our assumption was that using Apimast would reduce the overall cost of the disease on an EU farm. The results showed that Apimast could reduce the number of clinical cases on an average EU farm of 100 cows from 21 to 15, thereby reducing the cost by € 500 per year and the incidence of subclinical mastitis from 19% to 12%, thus reducing the cost by € 1,105 per year. The annual average cost of the disease per cow with Apimast would decrease by € 22, so with Apimast using the cost would be € 94, and without using it € 116. The annual cost of mastitis prevention measures on an average EU farm of 100 cows would be € 200 cheaper when using Apimast. The total annual cost of the disease on an EU farm of 100 cows would be € 212 / cow when using Apimast and € 236 / cow without Apimast, which means a savings of € 24 per cow per year will be made with Apimast. In this research, we have proven that the use of Apimast is cost-effective. In addition, the use of Propolis in the treatment and prevention of mastitis is a very interesting environmental and public health alternative to the use of antimicrobials that is in reality absolutely necessary in today's world.