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master's thesis
CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2019. urn:nbn:hr:178:495013
Jurišić, Lucija
University of Zagreb
Faculty of Veterinary Medicine
Department of Microbiology and Infectious Diseases with Clinic

Institutional repository: Repository of Faculty of Veterinary Medicine

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Jurišić, L. (2019). CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:495013

Jurišić, Lucija. "CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:495013

Jurišić, Lucija. "CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:178:495013

Jurišić, L. (2019). 'CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 22 May 2024, https://urn.nsk.hr/urn:nbn:hr:178:495013

Jurišić L. CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2019 [cited 2024 May 22] Available at: https://urn.nsk.hr/urn:nbn:hr:178:495013

L. Jurišić, "CHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:178:495013

Metadata
TitleCHARACTERIZATION OF EMERGING BLUETONGUE VIRUS SEROTYPE 3 STRAINS IN THE MEDITERRANEAN BASIN BY NEXT GENERATION SEQUENCING
Title (croatian)TIPIZACIJA EMERGENTNOG VIRUSA BOLESTI PLAVOG JEZIKA SEROTIPA 3 NA PODRUČJU MEDITERANA KORIŠTENJEM METODE SEKVENCIRANJA SLJEDEĆE GENERACIJE (NGS)
AuthorLucija Jurišić
MentorLjubo Barbić (mentor)
Committee memberDean Konjević (predsjednik povjerenstva)
Committee memberZoran Milas (član povjerenstva)
Committee memberLjubo Barbić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Microbiology and Infectious Diseases with Clinic)
Zagreb
Defense date and country2019-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Bluetongue (BT) is a vector-borne viral OIE-listed disease of wild and domestic ruminants caused by bluetongue virus (BTV). BT has severe economic repercussions for the livestock industry due to direct losses caused by the infection but also due to indirect losses as a result of restrictions on animal trade. Since 1998, Southern Europe and the Mediterranean basin have experienced multiple incursions of different strains belonging to different serotypes and topotypes of BTV. In 2016 a BT outbreak occurred in Tunisia when the etiological agent was characterized as a novel western BTV serotype 3 strain. One year after the notification in Tunisia, BTV-3 outbreaks were evidenced in Sicily and Sardinia. Considering the history of the epidemiology of BTV in the Mediterranean basin and the absence of specific vaccines, a novel BTV-3 certainly represents a risk for the European livestock industry. Therefore, in this thesis several aspects were analyzed including the development of a specific and accurate real time RT-PCR for the detection of BTV-3 RNA, the analysis of BTV-3 spread by means of virological and serological methods within the particular emphasis on a genome characterization of BTV-3 using a next generation sequencing (NGS), a novel molecular diagnostic technique.
Abstract (croatian)
Bolest plavog jezika (BPJ) je vektorska bolest divljih i domaćih preživača koja se prenosi virusom bolesti plavoga jezika i koja se nalazi na listi bolesti Svjetske organizacije za zdravlje životinja (OIE). BPJ ima ozbiljne ekonomske posljedice za stočarsku industriju zbog izravnih gubitaka uzrokovanih infekcijom, ali i zbog neizravnih gubitaka kao posljedica ograničenja u trgovini životinjama. Od 1998. godine na području južne Europe i Mediterana došlo je do višestrukih izbijanja bolesti uzrokovanih različitim serotipovima i topotipovima virusa BPJ. 2016. godine došlo je do epidemije BPJ u Tunisu, kada je etiološki agens okarakteriziran kao novi soj virusa bolesti plavog jezika -serotip 3. Godinu dana nakon izbijanja u Tunisu, epidemije virusa bolesti plavog jezika serotipa 3 zabilježene su na Siciliji i Sardiniji. Obzirom na epidemiološku situaciju pojave virusa BPJ na Mediteranu i na odsutnost specifičnih cjepiva za novi serotip bolesti, navedeni serotip 3 virusa BPJ zasigurno predstavlja rizik za europsku stočarsku industriju. Stoga je u ovoj tezi analizirano nekoliko aspekata, uključujući razvoj specifičnog i preciznog RT-PCR-a u stvarnom vremenu za detekciju RNA serotipa 3 virusa BPJ te širenje serotipa 3 virusa BPJ pomoću viroloških i seroloških metoda s naglaskom na tipizaciju genoma serotipa 3 virusa BPJ korištenjem najnovije tehnike molekularne dijagnostike- sekvenciranja sljedeće generacije (NGS).
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:178:495013
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-11-11 12:03:23