The goal of every successful poultry industry is maintaining the health of the flock. But, young birds with the most prominent growth potential are the category that is the most prone to infection. Gastrointestinal tract is, because of its function, constantly exposed to various microorganisms, some of which are desirable, while others are harmful to the growth and health of an individual. In this paper we conducted research on the impact of astroviral infection to the serum proteins of turkey poults, with the goal of finding potential markers for the detection of pathological changes before the clinical onset of the disease. For this research we used serums of the 15 poults and spleen and small intestine samples of 5 poults, of Hybrid genetics, of 5 and 13 days of age. Previous diagnostics on the spleen and small intestine samplesm with metagenomics using next generation sequencing, showed the presence of astroviruses in 13 days old flock, while younger flock was negative for all viruses and it was used as a negative control for later serum proteome analyses. Algorithm from the computer programe “Proteome discoverer” was used for identification and relative quantification of serum proteins. To identify proteins and their roll in organisms we used STRINGdb v11.0, KEGG Pathways, REACTOME Pathways. Proteome analysis showed a significant difference between positive and negative flock. Among 171 serum proteins with significant abundance changes between healthy and infected turkeys, 68 of them were unique proteins, from which 46 of them were overexpressed, and 22 under-expressed. In conclusion, 68 proteins could be possible markers for diagnostics and follow up of astrovirus or other enterovirus or systemic virus infection.