Parvovirus infection in dogs was first described at the end of the previous century. Due to the high rate of mutations, new subtypes often emerge and become dominant in the dog population. For this research, a total of 78 faeces and rectal swabs of dogs were collected and processed at the Clinic for Infectious Diseases of the Department of Microbiology and Infectious Diseases with the Clinic of the Faculty of Veterinary Medicine, University of Zagreb. This sampling and collection of data on animals included the period from 1 January 2019 to 1 December 2020. All animals and dogs showed signs of acute gastroenteritis. Data 37 on nationality, medical history, clinical examination, and course and outcome of treatment were used for statistical analysis. The presence of canine parvovirus-2 was demonstrated by a polymerase chain reaction in faecal samples or rectal swabs. After determining the nucleotide sequence of the amplified segments of the viral genome, the presence of all three known subtypes of the CPV-2 virus was established, and the most common subtypes are CPV-2c (53.85%) and CPV-2a (35.9%). Additional anamnestic data showed that the CPV-2c subtype was more common in shelter dogs. The same subtype is more commonly responsible for infections in puppies, while the other two are common in older dogs. Assessment of the clinical picture, course and outcome of disease showed that there was no significant difference between individual virus subtypes. Immunoprophylaxis gives the same level of protection for all three subtypes. Finally, parvovirosis demands aggressive therapy regardless of the vaccinal status.