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master's thesis
Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2021. urn:nbn:hr:178:015883
Fajdetić, Marija
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Fajdetić, M. (2021). Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:015883

Fajdetić, Marija. "Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:178:015883

Fajdetić, Marija. "Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:178:015883

Fajdetić, M. (2021). 'Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:015883

Fajdetić M. Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2021 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:178:015883

M. Fajdetić, "Kvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:178:015883

Metadata
TitleKvantifikacija sojeva bakterije Escherichia coli uzgojenih simultano in vitro ili in vivo postupkom qPCR-a s ciljem dokaza klonalne selekcije
Title (english)Quantification of Escherichia coli strains cultivated simultaneously in vitro or in vivo using qPCR method with aim to prove the clonal selection
AuthorMarija Fajdetić
MentorŽeljko Gottstein (mentor)
Committee memberDanijela Horvatek Tomić (predsjednik povjerenstva)
Committee memberMaja Lukač (član povjerenstva)
Committee memberŽeljko Gottstein (član povjerenstva)
Committee memberJosipa Habuš (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2021-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Kolibacilozu u uzgojima peradi na manjem geografskom području ili na razini jata najčešće uzrokuje jedan ili nekoliko sojeva bakterije Escherichia coli koji pokazuju karakteristike klonalnosti. Cilj istraživanja bio je utvrditi da li se pojedini soj bakterije E. coli bolje i brže umnaža u in vivo i in vitro uvjetima prilikom simultanog umnažanja s drugim sojem zbog posjedovanja određenih gena virulencije, te da li potiskuje drugi soj. Za umnažanje sojeva koristili smo Brain Heart Infusion bujon, te komercijalne i SPF kokošje zametke, nakon čega su pojedini sojevi kvantificirani qPCR-om. Koristili smo četiri različita soja E. coli. In vitro pokus rađen je s uzorcima u triplikatu za svaki soj i kombinaciju, a u in vivo pokusu za svaki soj i kombinaciju korišteno je po pet komercijalnih i po pet SPF kokošjih zametaka. Praćeno je i ugibanje zametaka. In vitro pokus pokazao je da nema značajne razlike u simultanom umnažanju dvaju različitih sojeva u bujonu. S druge strane, prilikom simultanog umnažanja dvaju sojeva u kokošjim zametcima, soj koji je imao više gena virulencije se brže i bolje umnažao, te moguće potisnuo umnažanje drugog soja, što potvrđuje našu hipotezu. Temeljem dobivenih rezultata, moguće je da na sporije individualno umnažanje slabije patogenih sojeva u zametcima, pogotovo komercijalnim, potencijalno sinergistički djeluje i imunost domaćina, posebice vertikalno prenesena materalna imunost, što treba naknadno utvrditi.
Abstract (english)
Colibacillosis in poultry production, in a smaller geographical area or at the flock level, is most often caused by one or few strains of Escherichia coli that show clonality. Our goal was to determine whether a single strain of E. coli multiplies better and faster in in vivo and in vitro conditions after simultaneous inoculation with another strain due to the possession of certain virulence genes, and whether it suppresses another strain. Strains were inoculated in the Brain Heart Infusion broth, as well as in commercial and SPF chicken embryos. Afterwards, multiplication was quantified by qPCR. We used four different strains of E. coli. An in vitro experiment was performed in triplicate samples for each strain and combination of strains. Five commercial and five SPF chicken embryos per strain and combination were used for the in vivo experiment. Embryonic lethality was also monitored. An in vitro experiment showed no significant difference in the simultaneous multiplication of two different strains when propagated in the broth. On the other hand, during the simultaneous multiplication of two strains in the chicken embryos, the strain that had more virulence genes multiplied faster and better than the other strain, and possibly suppressed its multiplication, which confirms our hypothesis. Based on the results, we assume that slower individual multiplication of the less pathogenic strains in the embryos, especially in commercial ones, is probably synergistically influenced by immune mechanisms, especially vertically transmitted maternal immunity, what will be further investigated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:015883
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-13 09:39:37