Propolis has immunomodulatory, antimicrobial, antioxidant, analgesic and anti-inflammatory effects, which promotes faster wound healing. Important compounds in propolis are flavonoids, phenylpropenoids and phenolic acids such as coumaric acid, caffeic acid and ferulic acid. Caffeic acid phenethyl ester (CAPE) has an immunosuppressive effect by inhibiting bacterial growth, preventing cell division. The antioxidant action of propolis is stimulated by the high concentration of polyphenols and flavonoids. Non-alcoholic propolis extract shows antimicrobial effect on gram positive bacteria and gram negative bacteria. Propolis shows an antifungal effect against fungi. MTT cytotoxicity test demonstrated the safety of propolis against cells. Considering the results of the scratch test, 1.5%, 1%, and 0.5% concentrated propolis solutions achieved statistically significantly faster wound healing compared to wells treated with controls A, B, and C. Using the t test between control day 2 and propolis treated cells, a statistically significant difference in wound healing was found (p <0.0032). Over a time period of 3 days, there was a statistically significant increase in the growth of propolis-treated cells compared to the control group. Cells treated with 1.5% and 1% propolis solution had the best healing values. By its immunomodulatory action, propolis increases the release of vasoactive mediators such as arachidonic acid metabolites. ELISA isolation of interleukin 6 showed a statistically significant increase in IL-6 (p <0.0086) between wells treated with 1.5%, 1% and 0.5% propolis solutions and controls A, B and C for 3 days. Propolis increases the release of the cytokine IL-6 and growth factors such as PDGF and FGF. This has proven the immunomodulatory effect of propolis in promoting faster wound healing.